Case Study Report for Unknown #49 A new school for girls was opened in a larger village in Northern Pakistan‚ and the teachers were proud to have convinced many families in the surrounding villages to allow their daughters a basic education. The school was a success‚ and the 6-10 year olds girls quickly learned how to read and write and were even instructed in the sciences. However‚ 2 weeks before summer break‚ many of the girls and the teacher developed breathing problems that included severe coughing
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Abstract In an environment isolation procedure‚ experiments under categories‚ such as‚ morphology‚ physiology‚ antibacterial susceptibility‚ selective media‚ and biochemical provide results. Both the unknown isolate and members of the Micrococcus genus were shown to be obligate aerobes. By using staining methods‚ this proved that the organism is gram positive. Morphology‚ such as‚ orange pigmentation and coccus shape provide similarities to the Micrococcus genus. Physiological tests were shown to
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be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen. Hypothesis: For this experiment‚ antibodies will be placed in wells on an agar plate‚ surrounded by wells containing serum (proteins‚ antigens etc.). If the antibody interacts with the antigen of surrounding wells‚ then the presence
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diffusion Materials: Agar‚ Ruler Chemicals: Potassium Permanganate‚ Methylene Blue and Potassium Dichromate. Method: Obtain a pinch of potassium permanganate‚ methylene blue and potassium dichromate. Place each substance carefully on the surface of agar‚ carefully noting which is which so that the crystals are spread equally over the agar and not too close to the edge of the dish. Measure the diameter of the colored area immediately after adding the substance to the agar with regular 10-minute
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for their industrial production are Bacillus subtilis‚ Bacillus licheniformis‚ Bacillus amyloliquifaciens and Aspergillus niger General Lab Requirements: • Autoclave or pressure cooker • Hot Plate or Microwave oven • Nutrient Agar powder • Potato Dextrose Agar powder • Soluble starch • Weighing scales • Shaker • Spectrophotometer or colorimeter • Water bath (Temperature controlled) Materials per group of 4 students • Hand trowel or disposable spoons • Sterile pipettes (One each of
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of LB agar. We then put 250 µn of CaCl2 transformation solution into two micro test tubes for the purpose of changing the bacterium’s cell wall to allow the pGLO plasmid to enter more easily. Second‚ we placed one colony of E. Coli from our original plate into each of the micro test tubes. Subsequently‚ we extracted a loopful of pGLO plasmid with a sterile loop and then placed it in one of the micro test tubes. We incubated the tubes on the ice for ten minutes while we prepared four new agar plates
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microorganism referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce isolated colonies of the organism on an agar plate. The unknown test tube with the bacteria is flame sterilized using a Bunsen burner. Once the bacterium test tube has been flame sterilized‚ a flame sterilized inoculating loop will be used to gather
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semi-permeable outer membrane of E. coli would protect the bacteria from any antimicrobial properties of cilantro. A drop of cilantro juice and water in varying concentrations (1:10‚ 1:20‚ 1:40‚ 1:80) was added to a nutrient agar plate inoculated with S. epidermis and a nutrient agar plate inoculated with E. coli. The plates were incubated for 48 hours and then observed for a zone of clearing where the cilantro juice drop was placed. Cilantro was found to not display antimicrobial activity against either
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sure you flame the neck of the bottle to keep the procedure sterile. Place what you have withdrawn on to an agar plate. 5. then get your glass sterile spreader out of the packet making sure you only touch the handle and with the spreader spread the desired bacterial culture on an agar plate‚ making sure the lid of the culture container does not touch the surface‚ and that the lid of the agar plate is quickly replaced after this step has been carried out. 6. Sterilise the grabbing part and up the handle
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with gas: cracks/displacement of the agar) EMB- greenish metallic sheen ------ E. coli EMB- pink mucoid colonies -------- K. pneumoniae e.coli (left) k.pneumoniae (right) *TSI ALK/Ag-----Serratia marcecens‚ Salmonella paratyphi A red slant‚ yellow butt with cracks or agar displacement or bubbles if with:
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