temperature as temperature is lower their growth is also slower and temperature above 30ºC may cause mutation in petite and sporulation is highly inhibited. 6. Isolating Single Colonies: Done by streaking cultures out on an agar plate to dilute the cells on the surface of the agar‚ overlapping streaks‚ separation or distribution of cells to form isolated single colonies. 7. Estimating the Number of Yeast Cells in a Culture: How to differentiate between molds and yeast culture: Traditional methods:
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synthesis of ATP (Hertfordshire‚ 2011). Without ATP cellular functions fail and then die. Research Question: How does increasing the concentrations of Dettol Antiseptic Liquid from 0% to 100% affect the growth of Escherichia coli bacteria on the agar plates? Hypothesis: As the concentration of Dettol Antiseptic Liquid increases the inhibition of bacterial growth around the antiseptic disks also increases. Variables: |Type of variable
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Medical Mycology: Yeast and Pneumocystis| Reading Assignment:|Mahon‚ Chapter 10‚ pgs 215-219‚ Chapter 27‚ pgs 626-629‚ 634-636‚ Appendix B Lecture Notes: Medical Mycology| |U of W Tutorial on Mycology (organisms listed in objectives)‚ www.medtraining.org[->0]| _____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the
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I removed he swabs from their packets and swabbed the inside of my cheek and put them back in their packets. 3. I then lifted the lid of the TSA plate slightly at an angle to prevent airborne microorganisms from contaminating the agar and smeared the surface of the agar with the swab from the floor and labelled it ‘TSA floor’. I did the same for the swab from my cheek and labelled it ‘TSA cheek’. I repeated this process for the SDA
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Experimental Methods In this lab‚ we use the procedure from Chemically Defined‚ Complex‚ Selective‚ and Differential Media. We divide the agar plates that have the unknown in third‚ write our initials‚ and date on the bottom of the agar plate. We also divide the agar plates into quadrants‚ label each organism in their quadrant‚ write our initials‚ and date on the bottom of the agar plates. We flame the loop and let it cool for a minute‚ we remove the cap of each culture tube of organisms with our pinky while
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with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism. A gene is a piece of DNA that instruct for making
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the way physicians’ control/treat infectious disease within our bodies. In this lab‚ chemotherapeutic agents were evaluated by the disk-diffusion method. Chemotherapeutic agents are placed on the surface of a Petri plate containing Mueller-Hinton agar‚ which allows the agents to diffuse freely‚ and a growth medium is incubated over its’ surface. During incubation‚ the chemotherapeutic agents move from levels of high concentration‚ to low levels of concentration. To be effective‚ the agent must inhibit
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ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
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Experiment No. 03 Date: Name of the experiment: Aerobic plate count (APC) and total coliform count of supplied yogurt sample. Purpose: To determine the aerobic plate count and total coliform count of the supplied yogurt sample. Principle: Yogurt squeezed or extracted from milks are more or less acidic‚ depending on the product‚ the pH generally ranges from about 2.4-4.2 and contain sugar (4.7g)‚ fat
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effect of size on the effectiveness of diffusion Aim: To use agar blocks infused with 0.1 Molar sodium hydroxide (NaOH) and phenolphthalein to investigate the relationship between shape and surface area: volume ratio on the effectiveness of diffusion. Hypothesis: That for a cube of agar‚ the time taken for complete colourisation due to diffusion of HCl is directly proportional to the cubes volume. Materials: |A block of agar (10cm x 5cm x 3cm) with 0.1M NaOH and |1x 250mL beaker
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