uses of bacteria. Throughout the investigation a series of experiments will be conducted to determine if metals such as nickel and aluminium have a resistance to E.coli K12 over a sequence of generations. To complete the investigation four Macconkey agar plates were inoculated with E.coli k12 and four paper discs of aluminium and nickel that were soaked in metal salts were placed into the plates to see the resistance that would occur over. Once resistance had occurred‚ the zone of inhibition was scraped
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RESEARCH PAPER ON BIOLOGICAL ACTIVITY OF ACTINOMYCETES ................ ABSTRACT: The microorganisms are ubiquitous in nature. They are found in probably every environmental condition present on earth. Actinomycetes were originally considered to be an intermediate group between bacteria and fungi but now are recognized as prokaryotic organisms with high G+C (>55%) content in their DNA. Our project aim is to evaluate some biological activities of Actinomycetes like:
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the glass tube test and the agar-water gel test. In the glass tube set-up‚ two cotton plugs soaked in two different substances (HCl and NH4OH) were inserted into the two ends of the glass tube. The substance with the lighter molecular weight value (NH 4OH‚ M = 35.0459 g/mole) diffused at a faster rate (dAve = 25.8cm)‚ resulting in the formation of a white ring around the glass closer to the side of the heavier substance (HCl‚ M = 36.4611 g/mole; dAve = 10.8 cm). The agar-water gel set up was composed
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various petri dishes containing agar‚ E Coli and the slime mold Dg‚ and the pH level is changed‚ then the petri dishes containing pH 4 (potassium acid phthalate) and pH 5 (sodium and potassium phosphate) will grow fruiting bodies more rapidly than pH 7 (potassium phosphate) and pH 9 (potassium phosphate and sodium borate)‚ since slime molds have a tendency to move away from ammonia‚ they probably do not prefer basic solutions. METHOD Materials: 4 petri dishes with agar and E Coli Dg pH 4 – potassium
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in a color change of the medium from green to blue. Materials needed include simmons citrate agar slant tube‚ and a pure culture of bacteria. The starch hydrolysis test is used to determine the ability to hydrolyze starch. Bacteria use amylase‚ an extracellular enzyme to hydrolyze bonds linking the glucose subunits. Starch is detected by adding iodine to the medium. If the bacteria growing on the starch agar produce amylase‚ no color change will be seen around the bacterial growth because all of the
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was place on a rack then the stain were apply. B. From agar cultures. 1. A drop of water was place in the center of the glass slide. 2. With sterilized inoculating loop to obtain a minute of bacterial culture from the agar cultures 3. It then mix with the drop of water and then proceeds as broth cultures. SIMPLE STAINING TECHNIQUES Material: 1. 24 hours broth cultures of a. E. coli b. Staph. aureus 2. 24 hours nutrient agar slants of a. Bacillus subtilis b. Pseudomonas aeruginosa
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ratio is considered to be the most significant factor in triggering a cell to divide‚ and therefore‚ determining cell size. OBJECTIVES • Determine the extent and rate of diffusion into three different size agar cubes. • Calculate the surface area to volume ratio for each agar
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ANTIMICROBIAL ACTIVITY OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEABYSITUMBEKO LIWELEYA(s213459531)Research Proposal Submitted in fulfilment of the requirements for the degree of BTECHBIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | atNelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGYDUE DATE- 16th MAY‚ 2013.SUPERVISOR- PROFESSOR N. SMITH | | Contents ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA
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Brain break- what type of medium is blood agar- COMPLEX Differential vs selective vs differential AND selective media Differential- ingredient added that can be changed by certain bacteria in a recognizable way Blood agar Hemolysis Alpha- green Beta- yellow surrounding strep throat Selective Hemolytic Ingredient added that inhibits growth f many unwanted organisms Antibiotic-containing medium Selective and differential MacConkey agar – gram neg that ferment lactose Bile
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experiment. In part A of the lab‚ diffusion was demonstrated with two solids and an agar gel petri dish. One crystal of potassium permanganate and one crystal of methylene blue were placed on either side of an agar gel petri dish. The purpose of this experiment was to determine which of the crystals would diffuse across the gel more. So the question is‚ which solid would have a higher rate of diffusion through the agar‚ methylene blue or potassium permanganate? Diffusion is the movement of particles
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