the 2 tests: the glass tube setup and the water agar-gel setup. In the glass tube setup‚ two cotton balls were soaked in the solutions of hydrochloric acid (HCl) and ammonium hydroxide (NH4OH) and were simultaneously placed on both ends of the tubing.NH4OH had a lighter molecular weight of 35 g/mole which diffused at a faster rate of 24.8 cm and formed a white smoke near the HCl end that had the molecular weight of 36 g/mole. The water agar-gel setup was made up of a petri dish containing
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the glass tube test and the agar-water gel test. In the glass tube set-up‚ two cotton plugs soaked in two different substances (HCl and NH4OH) were inserted into the two ends of the glass tube. The substance with the lighter molecular weight value (NH4OH‚ M = 35.0459 g/mole) diffused at a faster rate (dAve = 25.8cm)‚ resulting in the formation of a white ring around the glass closer to the side of the heavier substance (HCl‚ M = 36.4611 g/mole; dAve = 10.8 cm). The agar-water gel set up was composed
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for specific organisms. For i.e. Mackonkey agar only grows on gran(-). If a sample is negative it grows and if it is positive it doesn’t grow. Last but not least Differential media relies on color change of the media itself. This color change highlights the bacteria if it has a specific trait. For i.e. If sugar ferments there will be a color change. III. Reagents: 1.Tryptone 2.Yeast extract 3.NaCl 4.KCl 5.MgCl2 6. MgSO4 7.Glucose/ Dextrose 8.H20 9.Agar( for solid) IV. Methods Grab an Erlenhymer
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General Purpose media is designed to grow most organisms and do not contain growth inhibitors. Standard Methods Agar and Blood Agar Bases are examples of general purpose media. Differential Media Differential media contain a component that allow an observable change when a specific chemical reaction takes place. Simmons Citrate Agar is an example of a differential medium. In Simmons Citrate Agar‚ there is a pH indicator that turns from green to blue when citrate is utilized as the sole carbon source
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Laguna. B. Maintenance of Microbial Cultures Bacterial isolated were subculture on slants of Nutrient Agar (NA). Incubation of the subcultured specimens for bacteria was done for 7-14 days in Isolation Room. C. Media Preparation for Assay Three tubes with nutrient agar were prepared. The cultures were inoculated onto their respective media by placing 0.2ml of each. The inoculated agar was then poured to individual sterile plates. D. Extraction from Leaves The extraction was done through
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The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain
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lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria on it to see if the bacteria obtained is gram positive or gram negative. My hypothesis is there will be a lot of bacterial growth on all of the plate. Materials and Methods -Petri dish containing nutrient agar -Cotton swabs -Sharpie A Petri plate containing nutrient agar was used in the experiment. A sharpie was used to section off four quadrants of the dish for
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TRIPLE SUGAR-IRON AGAR TEST Triple sugar-iron (TSI) agar test- designed to differentiate among the different groups or genera of the Enterobacteriaceae‚ which are all gram-negative bacilli capable of fermenting glucose with the production of acid a. Differentiation is made on the basis of differences in carbohydrate fermentation patterns and hydrogen sulfide production. To facilitate observation of carbohydrate utilization patterns- TSI agar slants contain lactose and sucrose (1%) concentrations
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5 drops of methyl pH red indicator was then added. During the Triple Sugar Iron Agar (TSI) test‚ A TSI slant was inoculated with bacteria at 37oC for 48 hours. Color changes and production of gas were then checked for. For the Phenyl Alanine Deaminase test‚ a phenylalanine slant was inoculated and incubated at 37oC for 48 hours. 5
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Makalah Biologi Percobaan Reaksi Hidrogen Peroksida dengan Katalase Penulis: Jancolin Yani Pendahuluan Seperti yang kita tahu bahwa hydrogen peroksida yang dihasilkan oleh sel-sel didalam tubuh kita akibat metabolism sel akan terpecah menjadi gas hydrogen dan oksigen dengan adanya keberadaan enzim katalase. Zat hydrogen peroksida sangat berbahaya bagi tubuh dalam kuantitas yang banyak oleh karena itu harus dipecah dan dikeluarkan. Sebagian besar zat hydrogen peroksida
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