quickly diffusion occurs is dependent on several factors‚ such as temperature‚ particle size‚ and the concentration difference on either side of the membrane. Testing the hypothesis relating to the effect of molecular weight to the diffusion rate‚ agar plate set-up was used to observe the diffusion of different substances. The methylene blue and potassium permanganate (KMnO4) was used and placed in the plate. Because the two substances have distinct colors‚ the measurement of the diffusion zone was
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was in order to test the many effects of diffusion and osmosis amongst four experiments. One such experiment was testing the effects of molecular weight on diffusion in relation to the use of Agar. The methods performed included the use of two acids‚ HCl and acetic acid. Both acids were placed into an Agar-filled dish and‚ over increments of 15 minutes‚ data collection was taken based off the diffusion rate and the diameter length of both the HCl and the Acetic Acid. The resulting factor was the
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____________________ ABSTRACT The effect of molecular weight on the diffusion of substances were tested through the use of agar-water gel with three wells placed in a petri dish. Drops of three different substances namely: Potassium permanganate (KMnO4)‚ Potassium dichromate (K2Cr2O7) and Methylene Blue were placed in the wells of the agar-water gel in the petri dish. The diameter of the diffused substance was noted every 3 minutes for 30 minutes. The Potassium permanganate (KMnO4)
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Digital image. Flickriver.com. Flickriver Viewer Software‚ 2007. Web. 3 Nov. 2013. An endospore stain of Bacillus cereus using malachite green. Digital image. Academic.missouriwestern.edu. N.p.‚ n.d. Web. 3 Nov. 2013. Hahn‚ Brittany. Phenyl Ethyl Alcohol agar. Digital image. Studyblue.com. STUDYBLUE INC.‚ 22 Apr. 2013. Web. 3 Nov. 2013. Reynolds‚ Jackie. Selective and differential media. Digital image. Www.microbelibrary.org). Kern Community College District‚ 12 Sept. 2011. Web. 3 Nov. 2013. Rose‚ Scott
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Unable to read the Gram Stain at first it was later confirmed that Unknown #7 was a gram negative bacteria. When introduced to Starch Unknown #7 produced no zone of clearing showing that it did not contain the enzyme amylase. When introduced to the Lipid Unknown #7 did produce a zone of clearing indicating that it did contain the enzyme lipase. When introduced to Casein Unknown #7 produced no zone of clearing indicating that it did not contain the enzyme protease. When streaked onto the Gelatin‚
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he root system is developed in the soil since soil can help establish their strand and protect the fragile roots from stressful conditions such as drought‚ nutrient deficiency‚ unfavourable temperature‚ etc. Hydrotropism is a directional growth response exhibited in terrestrial plants in which water or water vapour is the orienting stimulus. In hydrotropism the root caps perceive and respond to moisture gradient of the soil simultaneously which will then regulate the directional growth of the root
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Permanganate‚ Potassium Dichromate‚ and Methylene Blue.1 Arantxa Alex Carpio Group 1 Sec. X – 4L March 24‚ 2015 ABSTRACT The effect of molecular weight and time on the rate of diffusion was determined using the agar-water gel test. A petri dish of agar-water gel with three wells was prepared and a prepared solution of each substance was dropped on each well; one with potassium permanganate (KMnO4)‚ the other with potassium dichromate (K2Cr2O7)‚ and the last one with methylene blue
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human body samples by culturing them on the appropriate media using aseptic transfer techniques. Materials • Distilled water • Test tube • 6 Unopened packages of 1 sterile cotton swab • 2 sterile nutrient agar Petri dishes • 1 sterile blood agar Petri dish • Incubator • Refrigerator • Bunsen burner • Gas connection • Plastic tubing • Inoculating loop • 12 sterile glass slides • Wax pencil • Igniter • Crystal violet dye
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substances and absorb toxic metabolites this organism is often grown on media with rich concentrations of blood‚ or charcoal. ("Pertussis."). Two different solid cultures that are used in growing B. pertussis are Bordet-Gengou agar (BGA) and Regan-Lowe agar (RLA). Bordet- Gengou agar contains potato starch and is peptone free‚ also an inhibitory substance. It also contains glycerol as a stabilizer‚ and an antibiotic‚ such as penicillin‚ to restrain gram positive organisms from growing‚ although the
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2 were Pseudomonas aeruginosa. One of each was used to confirm. Finally only MTCC cultures were used to prepare inoculum. Inoculums Preparation Primary isolation was initiated by nutrient agar plating and subsequently transferred to selective media like Pseudomonas Isolation Agar‚ MacConkey Agar and EMB agar for better isolation. Using sterile inoculation loop colonies of the test organism are transferred to 5ml of sterile nutrient broth and incubated at 37 0C overnight for 24hrs. Then this bacterial
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