"Agar" Essays and Research Papers

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    The effects of black tea on the growth of E. coli bacteria cultures Kierstin Barker‚ Melissa Bischak‚ Jackie Tyszkiewicz‚ Errin Enany September 25‚ 2012 Abstract: This study was carried out in order to investigate whether black tea has antimicrobial properties as stated in Steven Johnson’s The Ghost Map. If tea does have antimicrobial properties‚ then it could aid in warding off waterborne diseases. We believed that if black tea is steeped in boiled water‚ then the amount of bacteria exposed

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    glass tube test and the agar-water gel test. In the glass tube test‚ two cotton balls were soaked in two different substances‚ one in hydrochloric acid (HCl) and the other one in ammonium hydroxide (NH4OH). The cotton balls were inserted into the two ends of the tube at the same time. After some time‚ a white smoke was observed to evolve nearer the cotton ball soaked in HCl. This indicated that NH4OH‚ which is lighter‚ diffused faster than HCl. The next test was the agar-water gel test. It was composed

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    Klebsiella Pneumoniae

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    Cyra Vose Klebsiella Pneumoniae #34 MCB 2010C Friday 9:00am Introduction: On November 16‚ 2012 at 0900 I was given case #34. Mr. Joe Blough presented with a sudden high fever (103 degrees Fahrenheit or higher) and mentioned he felt dizzy and had the chills. During this visit Mr. Blough seemed to be coughing and producing sputum. This sputum was thick and had some blood present. I had my assistant collect a sample of the sputum so we could run some tests and hopefully correctly diagnose Mr. Blough

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    this study. All procedures‚ unless otherwise noted‚ have been followed and performed exactly as stated in the laboratory manual Leboffe & Pierce(1). Using the Quadrant Streak technique the unknown and ubiquity were streaked onto a trypticase soy agar (TSA) plate. This method was performed as stated in the Leboffe & Pierce lab manual(1). The TSA plates were incubated at 30 degrees Celsius for 48 hours. This process was repeated until isolated colonies appeared. At this point cell morphology was

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    Root Canal Lab Report

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    All strains were provided by Ondokuz Mayıs University‚ the Faculty of Veterinary Medicine and Faculty of Medicine. Agar diffusion test The microbiological assays were performed under aseptic conditions inside a class 2 cabinet (Danlaf VFRS 1806‚ Denmark) previously sterilized. All testing procedures were carried out by a single operator using sterile instruments. Agar diffusion test (ADT) was used for determination of antimicrobial activities of test materials. The microorganisms inoculated

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    negative control‚ 15 seconds‚ 30 seconds‚ 45 seconds‚ and 60 seconds). Aseptic technique was critical during each transfer of agars. Plain 50 agar was poured equally to each plate labeled. A cotton end of the applicator stick was wet with the liquid culture of bacteria‚ Serratia marcescens‚ and applied on the surface of agar the same way it was carried out to cover the agar gradient in the first part of the experiment. Plates were prepared for the UV exposure. It was in this step that each plate

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    his experiment was done to find out if garlic is effective in killing bacteria. This will help us understand the effectiveness of home remedies such as the use of natural herbs (including garlic) for medicinal purposes. Hypothesis Garlic will kill bacteria and prevent its further growth. Background Herbs and spices have traditionally been used in cooking as they add flavor to our food. These herbs and spices have also been known to many cultures for their medicinal properties. The oils that are

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    For the completion of this experiment the procedures were guided with the Rainbow Transformation1 lab manual. An Escherichia coli bacterial reference plate was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells

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    Difco Manual

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    Difco & BBL Manual Manual of Microbiological Culture Media Second Edition Editors Mary Jo Zimbro‚ B.S.‚ MT (ASCP) David A. Power‚ Ph.D. Sharon M. Miller‚ B.S.‚ MT (ASCP) George E. Wilson‚ MBA‚ B.S.‚ MT (ASCP) Julie A. Johnson‚ B.A. BD Diagnostics – Diagnostic Systems 7 Loveton Circle Sparks‚ MD 21152 Copyright 2009 Becton‚ Dickinson and Company 7 Loveton Circle P.O. Box 999 Sparks‚ Maryland 21152 ISBN 0-9727207-1-5 All rights reserved Printed in the United States of America AOAC

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    unknown was inoculated into a TSI slant by stabbing the specimen into the butt using a needle and then streaking on the surface of the agar. The sample was allowed to incubate for 24 hours and read for results. The next test was the citrate utilization slant which examines the ability for a bacteria to use citrate as the sole carbon and energy source. The citrate agar tube was inoculated with the bug by using a needle and then streaking over the surface of the slant. Upon an incubation period of 48

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