Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate
Free Bacteria Staining
Using Selective Media to Monitor the Fermentation Process of Cabbage and Cucumber‚ for Fourteen Days Abstract: Introduction: The goal of the ecological succession lab is to demonstrate succession with the fermentation of cabbage and cucumbers. The cabbage will ferment into sauerkraut and the cucumber will ferment to pickles during fermentation process that will changes the species structure and the community of time. During the fermentation process we watched the pH become more acidic‚ since
Premium Bacteria Acid Microbiology
Materials: A. Formulation of Hypothesis a. Apparatus - glass tube - cotton balls b. Substances - hydrochloric acid (HCl) - ammonium hydroxide (NH4OH) B. Testing the Hypothesis Concerning the Rate of Diffusion a. Apparatus - petri dish of agar-water gel with three wells b. Substances - potassium permanganate (KMnO4) - potassium dichromate (K2CR2O7) - methylene blue III. Procedure: A. Formulation of Hypothesis 1. Fasten the glass tube to a ring stand. 2. Simultaneously
Premium Diffusion Molecular diffusion Chemistry
aureus nasal carriage rates were found 28% (41/144) in normal population‚ and 31.5% (12/38) in hospital laboratory personnel. Materials: * Mannitol salt agar (MSA) plate – Mannitol is a carbohydrate that can be used by some bacteria as a nutrient. The use of mannitol is important in identifying Stapphylococcus species. Mannitol salt agar contains 7.5% sodium chloride (salt) whereas‚ most media contains about 0.5% sodium chloride. Organisms that cannot tolerate a high salt concentration will not
Premium Staphylococcus aureus Staphylococcus Growth medium
Universita Jambi merupakan salah satu universitas negri yang ada diprovinsi jambi‚ yang berdiri pada tahun 1963 tepatnya dibulan Maret tanggal 23. Gedung pusat UNJA berlokasi di desa mendalo‚ kecamatan Jaluko‚ Kabupaten Muaro Jambi‚ Provinsi Jambi. UNJA pusat merupakan universitas yang tidak hanya memiliki gedung-gedung yang digunakan untuk aktivitas perkuliahan‚ tetapi juga merupakan satu-satunya universitas yang berada di Jambi yang memiliki hutan yang masih cukup terjaga kondisinya‚ karena sebagian
Premium
nothing out of the ordinary except dehydration from the diarrhea. Lab tests showed that he had 13‚000 white blood cells/microliter of blood‚ and a methylene blue stain of his feces showed abundant neutrophils. His stool specimen was plated on MacConkey agar and several colonies on the plate turned bright pink after 24 hours. Based on symptoms and the lab results‚ he was treated with trimethoprim-sulfamethoxadole and intravenous fluid therapy. He improved quickly and was released after 24 hours.
Premium Escherichia coli Bacteria Microbiology
Today‚ certain antibiotics are used in fighting certain diseases caused by pathogenic bacteria such as Salmonella enterica and Escherichia coli. However‚ due to the improper usage of these antibiotics and the increasing antibiotic resistance of the bacteria‚ a lot of the medicines commonly used before to fight the same bacteria are now considered ineffective and because of it‚ scientists try to find ways such as discovering new antibiotics or recommending to the citizens some procedures and ways
Premium Bacteria Antibiotic resistance Escherichia coli
identify their bacteria. During the process of identification‚ the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB)‚ TSI (Triple Sugar Iron agar)‚ Phenol red sucrose‚ the SIM test‚ H2S by SIM‚ IMViC (indole‚ motility‚ voges-proskauer‚ and citrate)‚ Urease (urea broth)‚ PDase (Phenylalanine Deaminase)‚ Lysine Decarboxylase‚ and Ornithine Decarboxylase. Colonial morphology on EMB was used to
Premium Escherichia coli
Doctors use this to determine which antibiotics are needed to treat infections. b) The test is used to see how the antimicrobials impregnate on the disks are then placed on a plate which then are incubated to grow more bacteria it also allows the agar to diffuse over. Then a concentration gradient is created‚ if there is a zone of inhibition the the disks will show the growth being
Premium Bacteria Antibiotic resistance Microbiology
dichromate is an orange inorganic chemical reagent and methylene blue is a chemical compound commonly used for staining because of its color blue. These three substances have 158 g/mole‚ 294 g/mole and 374 g/mole‚ respectively. A jelly-like substance‚ agar-water gel in a petri dish was used as a medium for diffusion since water and air‚ as a medium‚ are not controllable
Premium Chemistry Potassium permanganate Oxygen