coli (yellow) Red- no color change – negative test Yellow – color change – positive test 6. MaConkey’s Agar Positive control - E.coli Selective for negative gram stain . Differential for organism that could ferment lactase Growth pink – positive No growth – negative 7. Gelatin Hydrolysis ( Gelatinase) Positive control – P.aeruginosa Liquid –positive test Solid - Negative test 8. Blood agar Positive control – S. aureus A. Betahemolysis B. alphahemolysis C. gammahemolysis 9. FTM *broth –
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stained and cell visualized by making use of contrast difference between backgrounds and cells‚ it is called negative staining. Simple staining Salmonella anatum obtained from liquid broth and agar plate was stained with methylene blue‚ safranine and crystal violet dyes respectively. Sample taken from agar plate is much more concentrated than sample from broth. We expected to observe the rod-shape for these bacteria and see that this is actually rod-shaped. Some parts of prepared smears were difficult
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will be done to determine the species of bacteria. It is predicted that Pseudomonas syringae will be the pathogenic bacteria. Materials and Methods: The bacteria from a rotten tomato was streaked on nutrient agar plate. The different colonies were then isolated and grown on nutrient agar slants. Slices of healthy tomato were inoculated using the grown bacteria (one type of bacteria per slice) and‚ there was a control which was not inoculated. The slices of tomatoes were allowed to incubate but
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plasmids and arabinose. -The purpose to bacterial transformation is to insert DNA into bacteria to change it’s traits. Materials: Quantity: E. coli starter plate 1 Poured agar plates 4 Transformation solution 1 LB nutrient broth 1 Inoculation loops 5 Pipets
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followed when writing scientific reports. Scientific writing is typically written in the passive voice. The pronouns "I"‚ "We" and "They" are not typically used.. For example‚ instead of writing "I used a TSA agar plate to isolate my unknown‚" it is customary to write‚ "A trypticase soy agar (TSA) plate was used to isolate the unknown." It is also customary to write in the past tense for most of the report. This includes the introduction‚ the summary‚ the description of the materials and methods
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plants and Chang (1993) reported that the world mushroom biodiversity counts 1.5 million species. Summary Abstract 1_ Prior knowledge A) Bacteria 1) Salmonella 2) Shigella B) Salmonella Shigella Agar C) Moringa Oleifera a very useful tree D) The solar disinfection with PET bottle 2_ Study of different methods to purify water A) Water filtration 1) Study of filtration system with simple and usual things 2) Study of flocculation with Moringa
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GROUP No.: | DATE: 02/14/13 | | NAME: | | EXPERIMENT No.25BACILLUS: Diagnostic Tests | | ASCOLI’S THERMOPRECIPITIN TEST Purpose: used to identify anthrax bacilli in animal hides and meat. Principle: This test was designed to detect B. anthracis antigens in the tissues of animals being utilized in animal by-products and thereby to reveal when these products contained ingredients originating from animals that had died of anthrax. The thermostable antigens involved are common to other
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by the high amount of colonies observed on Horse Blood Agar (>108/L)‚ mild amount of protein (30 mg/mL) and very high amount of red blood cells (Large+++) found in the urine. The bacterium identified in this specimen is Pseudomonas aeruginosa. This is confirmed by involving many tests and observation‚ such as Gram stain‚ oxidase test‚ catalase test and the observation of colony morphology on Horse Blood Agar‚ MacConkey agar and Tryptic Soy Agar. Pseudomonas aeruginosa is an opportunistic pathogen‚
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Imperata cylindrica as an Antibacterial Agent for Escherichia coli INTRODUCTION a. Background of the Study We all know that bacteria are everywhere. Bacteria are microscopic organisms whose single cells have neither a membrane-enclosed nucleus nor other membrane-enclosed organelles like mitochondria and chloroplasts. One example of bacteria is E .coli is a bacterium that is commonly found in the gut endotherms. E. coli and related bacteria constitute about 0.1% of gut flora‚ and fecal-oral
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amylase on the breakdown of starch. MATERIALS These were the materials used: four starch/ agar plates‚ a marker pen‚ 1mm graph paper ruler‚ 8mm cork borer‚ forceps and template for cutting holes‚ 1% Amylase‚ water‚ incubator set at 5‚ 20‚ 40 and 60 degree Celsius. METHOD This was the experimental procedure carried out: the materials above were collected. Six wells were cut using cork borer in the agar which was in the plate and removed the bored one with forceps. The incubate temperature‚ the
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