Plant Tissue Culture 151 Chapter 9 Plant Tissue Culture Techniques Lorraine Mineo Department of Biology Lafayette College Easton‚ Pennsylvania 18042 Lorraine Buzas Mineo (B.S.‚ Muhlenberg College; M.A.‚ Duke University) is a lecturer in the Department of Biology‚ Lafayette College‚ and has taught botany since 1978 and supervised the General Biology Laboratories since 1970. Research interests in physiological and forest ecology have culminated in several publications. Other interests include
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CORE BIOLOGY PRACTICALS You will need to know these practicals as the exam board may ask you questions based on them. Below is a summary of each one. Name of practical and independent & dependent variables Effect of caffeine on Daphnia heart rate Independent: caffeine concentration Dependent: heart rate of Daphnia Measuring the content of Vitamin C in fruit juice Independent: fruit juice Dependent: volume of juice required to decolourise 1cm3 of DCPIP The effect of temperature on cell membranes
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materials used in this study were Duranta leaves‚ Fusarium sp.‚ petri dish for the containment where cultures of Fusarium sp. were grown. Ethanol for extracting the Duranta leaves. Erlenmeyer flask for containment of the concentrations. Potato Dextrose Agar (PDA) served as the food of the fungus. Gloves‚ face masks and lab gown were also needed for laboratory safety rules. Preparation of Different Concentrations and Treatments Fresh Duranta leaves were collected for extraction. Leaves of Duranta
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to combat bacteria. Hypothesis If bacteria is exposed to garlic‚ then the garlic will to abolish the Escherichia coli‚ because the Escherichia coli (E. coli) is usually a weak bacteria. Procedure Have three petri dishes prepared with blood agar‚ and three test tubes with 100 milliliters of milk. Label three test tubes‚ “A‚” “B‚” and “C.” With a toothpick‚ add a small amount of the E. coli specimen to tube “B"; shake the test tube to mix throughly. For test tube “C‚” add the same amount
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Taste discrimination in young and old Drosophila melanogaster Introduction The ability to discriminate taste is critical for the survival and nutrition of an organism (Sellier et al.‚ 2010‚ Gordesky-Gold et al.‚ 2008). Many animals rely on their taste organs to detect and discriminate various types of taste such as sweet or bitter (Weiss et al.‚ 2011). When these organs come in contact with a food source‚ signals are sent to taste receptor cells. The activation of certain taste receptor cells by
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unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The initial streak-plate procedure was performed and placed in the incubator at 37◦C for 24-48 hours. Upon observing the growth on this plate‚ it is fairly obvious that one of the organisms is Serratia marcescens
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Biology Assessment: Influenza Structure: Viruses are non-cellular obligate intracellular parasites‚ requiring a living host cell in order to reproduce. A developed viral particle (virion) lacks the metabolic machinery of cells‚ containing just a single type of nucleic acid (DNA or RNA) encased in a protein coat or capsid. Viruses can be distinguished by their structure and by the nature of their genetic material (single or double stranded DNA or RNA). Viruses that affect humans are more difficult
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Question: How does the size of the cell affect its efficiency in exchanging substances through several ways‚ like diffusion? Aim: To plan and carry out an investigation to show the relationship between volume/Cm3‚ surface area‚ and diffusion using agar cubes measured in time/s; and to demonstrate‚ using diffusion‚ why the size of cells is limited‚ keeping the room temperature and pressure constant. Hypothesis: I expect to find that when the surface area to volume of a cell reaches a certain level
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17th‚ October 2010 Margarita SA: VOL RATIO AND AGAR BLOCKS Conclusion: For this experiment the agar jelly had to be cut in to five different block sizes‚ after that has been done we had to add all five blocks in to the test tube with the acid and time to see how long it would take for the colour to change from a pinky purple to clear. From this experiment I learnt that the bigger the size of the jelly is the
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Agar.io is a addictive multiplayer action packed game developed by Matheus Valadares. In the game you are a cell and your goal is to gain as much mass as you can by consuming both pellets and smaller cells without being swallowed by bigger enemy cells. You can either play a deathmatch where everyone is the enemy‚ in a party with your friends or as a colored team that must consume other colors accept for your own. While playing the game‚ there is no set score‚ players have to respawn when all of
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