Cloning of plasmid pUC19 in E.coli bacteria Introduction One aspect of the DNA cloning experiments that is carefully considered is the selection of cloning vectors. A variety of vectors have been created‚ each being suitable for a particular use. One common vector used in laboratories is a plasmid called pUC19. It is 2686 base pairs long and possesses an origin of replication which allows the production of over 100 copies in a competent E.coli cell. It possesses a multiple cloning site (MCS)
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what optimum combination of ingredients would best increase efficiency in soil productivity to equal the result of hydroponics (Cultivation of plants in nutrient solution rather than in soil). Through research our group came to the conclusion that agar‚ pearlite and mulched grass could all greatly contribute to enriching the nutrient of the soil. We predict that one of the treatments‚ using the above listed ingredients‚ will have a greater growth rate than the controlled plant strictly using hydroponics
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yang berarti kayu. yang dalam fengshui diartikan bahwa: • Logam dapat memotong kayu • Logam dapat mencair seperti air • Air dapat menghidupkan kayu Makna : Logo ini menggunakan desain minimalis (simple) dimaksudkan agar mudah ditangkap dan dimengerti dalam waktu yang relative singkat. Serta cukup mudah diingat karena keunikannya‚ yaitu pada pemilihan initial O pada huruf O dalam logo mizone ini digunakan sebagai simbol pictografi yang berfungsi untuk mengidentifikasi
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does magnetic field have on the formation of Liesegang rings (crystal growth in gel medium) on elements of ferromagnetic‚ diamagnetic and paramagnetic nature like Cobalt‚ Nickel‚ Copper and Manganese in their chloride compounds when performed in an agar medium? Lokesh Chandnani Candidate Number – 049369 – 0003 Supervisor: Mr. Jaydip Chaudhuri The Aga Khan Academy‚ Hyderabad Session: May 2015 Word Count: 3798 1. Abstract Liesegang rings phenomena is a special type of formation that occurs in
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A2 BIOLOGY CORE PRACTICAL SUMMARY Name of practical Independent & dependent variables Observing patterns by Ecological sampling Random sampling Systemic sampling Other variables to be controlled Abiotic factors e.g. light‚ temperature‚ soil water‚ humidity‚ O2 concentration‚ pH‚ aspect‚ slope angle equipment Gridded Quadrat Tape measure Point quadrat Pitfall trap Sweep Net Pooter Tullgren funnel Baermann funnel Method and outcome Several methods. 1 random sampling = set up grid using
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yang sangat keras untuk dapat mengembalikan kepercayaan tersebut. Dan lokasi dari high reach ini juga masi sangat minim sehingga mereka ingin mencari pihak yang berminat untuk melakukan franchise agar high reach dapat lebih berkembang lagi‚ terutama yang berlokasi pada gedung perkantoran agar memudahkan para orangtua yang bekerja dalam memonitor anak
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organisms possess this trait is useful in microbial identification schemes. The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. Materials: 18-20 nutrient hour agar slant culture of Staphyloccus aureus 4 day old nutrient agar slant culture of Mycabaterium smegmatis Hot plate and beaker Inoculating needle and loop Bibulous paper Carbolfuchsin Acid- alcohol Methylene blue Staining procedure: Fill beaker 2/3 full with water. Place beaker
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Discussion: For the Feulgen’s/Schiff’s Stain that tests for DNA‚ the samples that contain DNA will show up under a compound light microscope as a magenta color while samples that do not contain DNA will not show up as magenta. A sample of bacteria from an agar plate was used as a control for the DNA test‚ and it showed up as magenta from the Feulgen stain; this confirms that the test accurately indicates the presence of DNA. The results show that samples ALV-188-23 and ALV-843-20 contain DNA‚ which means
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petri dish. I cut three whole in the padding and layered four pads to leave the empty space in the middle. I placed the plywood panels on the top and bottom and secured the bottom and left the top unsecured for access. I made 2 liters of agar and poured it evenly into 12 petridishes with an area of 18.096 cm2. Each Petri dish was inoculated with an inoculation tube using sanitation methods and intending to keep the amount of bacteria added constant. The bacteria was then set into the sound
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2010 When we initially started the bacteria antibiotic experiment on February 10‚ 2010 our team selected e-coli and Rifampicin (Rb5) to find out the level of resistance e-coli would build towards this antibiotic. When we first checked our agar dish on February 15‚ 2010 we noticed two circles for the zone of inhibition the small circle was 1.5 cm and the medium circle was 3.00 cm. Two days later when we checked it again the small circle had stayed the same at 1.5 cm but the medium
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