growth of bacteria on Agar plates Hypothesis: That the bacteria will grow in colonies throughout the agar plates except for the one with the anti-biotic loop because some will fight off the bacteria. Method: (Method taken from prac sheet) Plate 1: Use the swab to cover your entire agar plate in your bacteria. You only need one swab of bacteria but be careful to cover the entire surface of your agar in a layer of bacteria. Carefully place an Antibiotic Mastring on top of the agar (use tweezers) in the
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I. Product Background A. Introduction In this highly luxurious extravagant world‚ food and other beverages has always been one of the things which maintain the mainstream of life. Aside from being one of the basics for survival‚ gatherings are also made perfect by food preparation and stress is now often associated with food. This activities show that food intake nowadays is far different from the conventional times. Furthermore‚ this lavish lifestyle most of the people have results in an
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Isolation and Purification of Lyngbya majuscula on Nutrient-enriched Agar Plates A Special Problem Isolation and Purification of Lyngbya majuscula on Nutrient-enriched Agar Plates ABSTRACT Lyngbya majuscula samples were obtained from the Phycology Laboratory stock culture of the UPV Institute of Aquaculture. Five (5) mm fragments of these were inoculated into agar plates that used 1% agar concentration enriched with varying concentrations (1.0%‚ 1.5%‚ and 2.0%) of Hughes‚ et
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Blue on Agar Plates at Different Temperatures Abstract: Diffusion is the movement of particles from an area of high concentration to an area of low concentration wherein substances are independent‚ rapid and in random motion which frequently collide with each other in the sides of the container. This study aimed to assess the influence of temperature on the diffusion rate of methylene blue on agar plates. To determine the relationship between the two variables‚ five set-ups of agar plates were prepared
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Microbiology Instructor: Dirk VandePol Date: 6/21/2013 Streak Plate Isolation for Obtaining Pure Culture 1. When an agar plate is inoculated‚ why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time‚ propose is to isolate the unknown bacteria. Therefore‚ the first time to streak on the plate‚ there are million of bacteria on the loop. For that reason‚
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Agar From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Not to be confused with auger or augur. For other uses‚ see Agar (disambiguation). Culinary usage Mizuyōkan - a popular Japanese red bean jelly made from agar. Scientific usage A blood agar plate used to culture bacteria and diagnose infection. Agar or agar-agar is a gelatinous substance derived by boiling[1] a polysaccharide in red algae‚ where it accumulates in the cell walls of agarophyte and serves as the primary structural
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Agar and Media Preparation— Agar plates containing King’s B Agar were often used throughout the experiment to support growth of Pseduomonas fluorescens. A recipe was used that included a mixture of 10g Proteose Peptone #3‚ 1.5g Potassium Phosphate Dibasic (K2HPO4)‚ 30ml 50% Glycerol‚ ~965ml water and 20g agar. The mixture‚ post- autoclave‚ was left to cool and 5ml 1M Magnesium Sulfate (MgSO4) was added and created about 40 plates. King’s B Medium was made using the same procedure as the King’s B
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Materials and Methods: Inoculation of the unknown was done on a nutrient slant agar The nutrient agar was then incubated at 27°C for one week. The agar appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain
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Making Agar Cushions 1. Start with wearing gloves and swabbing them with 70% ethanol to keep the materials that will be used in the experiment sterile. 2. Wipe the work station with Wescodyne and paper towels. It is important to keep the sterile materials such as slides in petri dish‚ Pasteur pipette in container and forceps covered as much as possible. 3. Place four to five filter papers in the petri dish and drip few drops of water on the strips‚ this will maintain moisture in the agar cushions
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Unknown #11: Citrobacter freundii Tryptic Soy Agar Test (TSA): TSA is a basic medium that is most similar to nutrient agar. The agar contains carbon‚ nitrogen‚ sodium chloride‚ and agar. This allows for the growth of a large variety of microorganisms to grow and ferment in the medium. Mannitol Salt Agar (MSA): MSA is a selective and differential medium that favors the growth of salt loving organisms. It is commonly used to distinguish the different species of Staphylococci. If an organism ferments
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