Aluminum foil Masking tape NA powder PDA powder Pentel pen Stirring rod Casserole Electric stove Pressure cooker/ autoclave Steps in Preparation of Culture Media: 1. Calculate the total amount of media needed for the experiment (15ml for plates‚ 5-7 mL for tubes). 2. Weigh the required amount of powder needed to dissolve in distilled water (based on the manufacturers specification in the container). 3. Dissolve the powder using the stirring rod‚ cover‚ cotton stopper and label.
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the height. * Diffusion in solids -4 agar plates -KMnO4 crystals -methyl orange -refrigeration -ruler The experiment requires 4 plates of agar due to his transparency and the colloid that forms when mixed with water. One pair of plates is labeled 4^0 C and the second pair RT (room temperature). After taking the lids of the first pair ‚using a tooth pick‚ a small crystal of potassium permanganate is placed in the center of the of the agar and the same amount of methyl orange. The
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7H2O‚ 0.2; CaCl2‚ 0.55; NH4Cl‚ 0.4; agar‚ 1.5. A stock solution of the dye was prepared and used for all studies. The sample collected was screened for CV dye decolorizing bacterial strains by inoculating 10 ml of wastewater into flask containing 100 ml of MSM broth. The flasks were incubated at 35°C under shaking conditions (120 rpm). After 48 hr of incubation period‚ 1ml of the culture broth was appropriately diluted and plated on MSM-CV dye amended agar plates. The morphologically distinct bacterial
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TSA (tryptic soy agar) media was placed in a different place to collect cells as well as printed with a thumb print from each member of the group. Incubation is used because each sample needed a certain temperature for the cells to grow. Inspection was used to look at each plate and see how many colonies are found. One TSA plate was placed wherever the student wanted in the building and the other was for each group member to press their thumb print in. The SBA (sheep blood agar) was used for a mouth
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broth‚ Blood agar plate‚ MSA plate‚ MAC plate‚ and the EMB plate. Before that we had done the 3 phase isolate which we had a possible of 10 points of achieving. In the isolation plate we had too take the sample of our unknown‚ which was the letter G. After we had done the 3-phase isolation plate we inoculated half of the plate‚ which was the S/D media. The Plate’s that we had provided too us were the BAP‚ MSA‚ MAC‚ and EMB. When we were successfully inoculated the S/D media the plates were put in
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Tanti Lim Thurs AM Unknown Project I. Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge
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SEROLOGICAL‚ CULTURAL AND MOLECULAR DETECTION OF BRUCELLA INFECTION IN BREEDING BULLS A THESIS SUBMITTED TO THE ANAND AGRICULTURAL UNIVERSITY IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF Doctor of Philosophy IN VETERINARY MICROBIOLOGY BY AMIT N. KANANI M. V. Sc. DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND-388001 (GUJARAT) 2007 Reg. No. 04-05194-2001 Dr. J. H. Purohit
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Experiment 6: Microbial Cultivation Objectives: To successfully cultivate microorganisms from different sources to medium. Materials: Broth‚ Agar‚ Sterilized cotton swab‚ Procedure: 1) Get your broth with cotton swab inside containing your bacteria. 2) Remove the cotton and flame sterilize the mouth of the testtube. 3) Get your cotton swab inside‚ flame sterilize again the mouth of the testtube then plug it with cotton. 4) Grab the inverted plated media and flame sterilize the
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LOVELY PROFESSIONAL UNIVERSITY CAPSTONE PROJECT REPORT TOPIC- ANTIMICROBIAL ACTIVITY OF DIFFERENT TYPES OF HONEY. PROJECT GUIDE- SUBMITTED BY- DR. AKSHAY GARG MOHIT KUMAR DEPT. OF BIOTECHNOLOGY REG. NO.- 10800037 ROLL NO- RB1R07B02 B.TECH BIOTECH.(8th sem.)
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CHAPTER-1 INTRODUCTION 1 INTRODUCTION The organisation can be defined as “the planned coordination of the activities of a number of people for the achievement of some common‚ explicit purpose or goal‚ through division of labour and function and through a hierarchy of authority and responsibility.” Organisations are not just means used by groups of people to achieve some goals. They present different images like‚ Organisations as machines Organisations as living systems Organisations
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