The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
Premium Streptococcus Microbiology Growth medium
means of both reproduction and dissemination of molds‚ since they are readily carried about by air currents. Septa = hyphal cross walls which divide the filaments into separate cells. Petri Plate (dish) = a special covered dish in which mold is cultured. Medium = a solid nutrient used for culturing. Agar = a non-nutrient thickening agent which is thicker than gelatin but still quite soft. Smear = a thin film of microbial cells on a microscope slide. Fixing = passing the smear through the flame
Premium Yeast Bacteria Fungus
strain Paenibacillus elgii TS33 25. The nutrient agar medium was prepared‚ sterilized at 121ºC‚ poured into the sterilized peteriplates and allowed to solidify under aseptic conditions. After solidification bacterial strain Paenibacillus elgii TS33 was spot inoculated on nutrient agar medium and incubated at 37ºC for 48 hours. After incubation the molten potato dextrose agar medium containing the spores of test fungus‚ was spread on the same plate and reincubated at 27º C for 3 days. RESULT Analysis
Premium Bacteria Microbiology Escherichia coli
CHAPTER I INTRODUCTION This chapter presents the background of the problem‚ main problem‚ the sub-problems‚ and hypothesis‚ significance of the study and the scope and limitations of the study. Background of the Problem Nile Tilapia (Oreochromis niloticus) is a very popular aquaculture species in the Philippines at present and considered as an “aquatic chicken” offering economical and social benefits mainly for rural communities. It also play vital role in terms of worldwide employment‚ however
Premium Bacteria Antimicrobial Agar plate
MASINDE MULIRO UNIVERSITY OF SCIENCE AND TECHNOLOGY P.O. BOX 190-50100 KAKAMEGA FACULTY OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES COURSE TILTE: INDUSTRIAL/FIELD ATTACHMENT COURSE CODE: SBL326 NAME: PAMELA K. MUKWEYI REG. No.: BTE/0517/08 DURATION: 9TH MAY – 20TH JULY 2012 SUBMISSION DATE: ATTACHMENT PLACE:CENTRE FOR MICROBIOLOGY RESEARCH- KEMRI Scope/purpose The Industrial Attachment program fulfils part of the requirement in pursuing the degree
Premium Blood Hematology Agar plate
ATCC 8007. The bacterium was activated in Man-Rogosa-Sharpe (MRS) agar medium (Difco‚ BD Diagnostic Systems‚ Maryland‚ USA) consisting of (g L1): peptone casein‚ 30; meat extract‚ 10; yeast extract‚ 6.0; sodium acetate‚ 5.0; ammonium citrate‚ 2.0; glucose‚ 0.2; magnesium sulfate‚ 0.2; manganese sulfate‚ 0.05; dipotassium phosphate‚ 2.0. The pH of the medium was adjusted to 6.5. The master cell bank culture was inoculated into agar plates and incubated for 48 h to produce the working cell culture. Stock
Premium Bacteria Agar plate
Abstract Introduction An antimicrobial is a substance that kills or inhibits the growth of microorganisms such as bacteria‚ fungi‚ or protozoans (Antimicrobial). Antimicrobial drugs either kill microbes or prevent the growth of microbes. Disinfectants are antimicrobial substances used on non-living objects or outside the body. Ginger Figure 1 : Ginger (Studies Reveal Ginger Lowers Colon Cancer Risk) Ginger is commonly used around the world and has been employed in the treatment‚ cure‚ and
Premium Antimicrobial Bacteria Petri dish
bacterial population from the normal throat flora. A streak plate method will be used to obtain a pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab sens test. A known Gram positive coccus bacteria
Premium Bacteria Microbiology Gram staining
Effect of chalepensin on S. mutans growth Fifty microliters of a S. mutans suspension (1 x 103 bacteria/ml) in BHI medium (Remel‚ Lenexa‚ KS) were transferred to flat-bottomed 96-well plates (Corning Incorporated‚ Corning‚ NY) containing serial dilutions (1:2) of 50 μl of chalepensin‚ or 1 μg/ml tetracycline‚ chalepensin-free vehicle (vehicle was similarly processed as with chalepensin extraction‚ but without plant material)‚ and culture medium controls‚ and incubated for 6 h at 37°C. Next‚ MTT was
Premium Enzyme Bacteria Immune system
setting them above the cone of the flame to make the loop red‚ then left them to cool in the test tube tack for streaking. The media included a liquid (YED media that was made by 3g yeast extract‚ 6g anhydrous dextrose‚ 6g agar‚ and 300 mL tap water). The liquid media was on a hot plate‚ then an oven mitt was used to carefully transfer onto 4 petri dishes at a tilted angle‚ with the petri dish lids at an angle as well to reduce airborne contamination. After the end of each pour—no more than 2/3 of the
Premium Laboratory glassware Agar plate Petri dish