An agar plate is a Petri dish that contains a combination of agar and nutrients that help microorganisms grow. The proper method of setting microorganisms on an agar plate is know as “streaking”. In order to streak‚ the microorganisms are placed on a sterile swab or metal wire‚ which is then dragged lightly against the agar solution‚ leaving behind the microorganisms. The amount of organisms is greatest at the beginning
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performing an enumeration (plate count) of the total viable bacteria in the rice salad on a general non-selective agar using either the pour or the spread plate method. To confirm that the outbreak had been caused by any B. cereus present in the rice salad a selective media agar‚ such as mannitol egg yolk polymixin agar (MEYP/MYP)‚ should be used. Once B. cereus has been confirmed a further enumeration of the B. cereus should be performed on the MEYP/MYP agar selective media plate to show whether the amount
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QUEENSLAND ACADEMIES FOR SCIENCE‚ MATHEMATICS AND TECHNOLOGY International Baccalaureate Diploma Program Extended Essay in Biology Is Tea-tree Oil effective in controlling and minimising the growth of the Escherichia coli Bacteria? A study of the effectiveness of a Natural Antiseptic Candidate Name: Lois Adrienne Dianne Soriano Dulay Candidate Session Number: 003072-032 Word Count: 3997 Supervisor: Ms Kate Barry Date of Submission: 25th July 2011 Acknowledgments Firstly I would
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Gain more knowledge about selective media and differential media. Practice use of the catalase test‚ coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of Mannitol salt agar‚ blood agar and MacConkey agar plates which must be used based on the components of the bacteria. The catalase test will be used to understand the difference in facultative anaerobic and groam positive from aero tolerant anaerobes. The coagulase test converts fibrogen
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morphology. Colonies can be classified according to their colour‚ form‚ elevation‚ margin and size. Pure cultures of microorganisms are those that are uniform and are of the same descendants of the same organism. The isolation of colonies by streak plate technique allows the obtainment of pure cultures which can be studied further. Good quality aseptic technique and proper sterilisation are a required to ensure that all samples produced are free from unwanted organisms that can lead to contamination
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Identification and Starter Culture Activity of Lactoccocus lactis MATERIAL & METHODS MATERIAL: Apparatus: 1. Capped test tubes 2. Test tubes holder stand 3. Beakers 4. Microscope 5. Incubator 6. Platinum loop 7. Microtitre plate 8. Durham tubes 9. Conical Flask 10. Measuring cylinder 11. China dish 12. Funnel 13. Spatula 14. Weighing balance 15. Autoclave 16. Aluminium foil 17. Petri dishes with lids 18. pH meter 19. Burner
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Techniques for Isolation of Pure Cultures Objective : i. To perform the spread plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate
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I first plated my unknown mixture on Tryptic Soy Agar (TSA)‚ Columbia Naladixic Acid (CNA)‚ and MacConkey’s Agar (MAC) plates. After 48 hours of incubation‚ it was unclear that two different bacterial colonies had grown on my TSA plate. Only one type of colony was evident. However‚ it was apparent that I had successfully isolated two different bacterial species by examining my MAC and CNA plates. Only one type of colony had grown on my MAC plate indicating a gram negative species‚ which I chose
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In the 2nd phase‚ chitin will then be dissolved in less than 40% of Sodium Hydroxide‚ and heated to 90celcius which will convert the Chitin into Chitosan upon dissolving. The third phase will take place over 3 days. The nutrient broth and nutrient agar will be prepared first. On the 2nd day‚ the bacteria broth will be prepared prior to an overnight culture on the same day using E-Coli and M.Luteus. On the 3rd day‚ the Anti-bacterial well diffusion test will be carried out. Finally‚ we will
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nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of each petri dish and evenly spread bacterial culture around the agar plate. Cover and allow the culture to soak into agar for at east 15 minutes. Using sterile forceps‚ carefully place one filter disk from designated sample into the middle of each
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