Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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39 agar plates‚ sterile cotton swabs‚ a concentrated amount of Escherichia coli (E. coli)‚ three different levels of antibiotic (Ampicillin: 1mg‚ 5mg‚ and 10mg)‚ a bunsen burner‚ metal tweezers‚ an incubator‚ 27 broths‚ and a ruler which should all be provided through your laboratory. For the first generation‚ you will be using 12 plates. Three of the plates will be labeled G1 control‚ three of the plates will be labeled G1/1 mg‚ three plates will be labeled G1/5 mg‚ and the last three plates will
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active dried yeasts (ADY). This experiment allowed the students to perform the plate count technique by serial dilution and two common methods‚ spread plate and pour plate to determine the colony forming unit (CFU) of yeasts A ten-fold dilution is used in this experiment‚ the sample is diluted until it reached the 10-9 dilution. Plating for spread plate started from 10-5‚ 10-6‚ 10-7 and 10-8 dilution while for pour plate‚ it started from 10-6‚ 10-7‚ 10-8 and 10-9 dilution. Having incubated inverted
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2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior to use‚ melt and hold at 50c in a water bath 5 tubes of 9.0 ml trypticase soy (TS) broth 1 ml sterile pipettes Pipette aids Methods: 1. We began the experiment by marking the plates and place them in order from 10^5‚ 10^6‚ 10^7‚ then have the blank dilutions ready in order from 10^-4
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organisms can easily be seen using differing types of agar‚ which creates an ideal environment for the organisms to form colonies‚ which are groups of hundreds of organisms that can be seen with the naked eye. In order to see individual microorganisms‚ it is necessary to use the magnification of a high-powered microscope. These techniques of microbiology are used in the following five experiments. The first experiment used Trypticase Soy Nutrient Agar (TSA)‚ which can grow a wide variety of organisms
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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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002141-0012 Nuba Jackson IB Biology Microbiology IA How effective is Lysol in the reduction of bacterial growth compared to Pinesol in reduction of E. Coli growth in agar at room temperature? Background Information: Pinesol and Lysol are both common household disinfectants that make very big commercial claims; both claim to kill 99.9 percent of bacteria. Lysol contains
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Plate Tectonics Designed to meet South Carolina Department of Education 2005 Science Academic Standards Table of Contents Plate Tectonics: The Beginning (slides 3 and 4) Layers of the Earth (slides 5 and 6) Standard 8-3.1 What are Tectonic Plates- movement? (slides 7 and 8) Standard 8-3.6 Tectonic Plate boundaries (slides 9-21) 1. Convergent boundary Ocean-continent (slide 10) Continent-continent (slide 11) Oceanic-oceanic (slide 12) Volcanism (slide 13) 2. Divergent boundary Sea-floor
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swabbed uniformly across a culture plate. Then a filter-paper disk‚ impregnated with the compound to be tested‚ is placed on the surface of the agar. The compound diffuses out from the filter paper into the agar. The concentration of the compound will be higher next to the disk‚ and will decrease gradually as distance from the disk increases. If the compound is effective against bacteria at a certain concentration‚ no colonies will grow wherever the concentration in the agar is greater than or equal to
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yeast and mold in a sample. It counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count‚ like pour plate method‚ spread plate method and most probable number method. The viable count is very specidic‚ as it represents the number of colony forming
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