A2 Practical Investigations Title: The effect of Temperature on the growth of Aspergillus oryzae Develop a Hypothesis This particular investigation is to discover how a range of temperatures effects the growth rate of the fungi Aspergillus oryzae. Most fungi’s tend to survive within the temperature range of 5-35oC‚ with the optimum depending on their normal environmental temperature. The fungi Aspergillus oryzae is heterotrophic which means they taken in their food from dead organic matter and cannot
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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CHAPTER ONE 1.0 INTRODUCTION Natural rubber is produced by over 2000 plants species and its main constituent is poly (cis-I‚4-isoprene).a highly unsaturated hydrocarbon. Since 1914 there have been efforts to investigate microbial rubber degradation: However‚ only recently have the first proteins involved in this process have been identified and characterized and have the corresponding genes cloned. Analysis of the degradation product of natural rubber and synthetic rubbers isolated from various
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Yellow | ColourlessMedia: Pink | ColourlessMedia: Yellow | Pink-RedMedia: Pink | ColourlessMedia: Pink | E. coli grown in EMB agar: As we can see from the table above‚ the streak of E. coli on a plate with EMB agar showed a metallic green sheen where E. coli was present. EMB stain is selective for gram-negative bacteria. It is made using 6:1 Eosin and Methylene Blue. EMB agar is a differential media and inhibits the growth of gram-positive bacteria while also using a colour indicator to tell the difference
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TSI test because there is no pink color for Urea test and no black color on the bottom of the tube in TSI test. Then‚ follow three tests results are all positive. That is deep blue color for citrate test‚ clear zone surrounding growth for skim milk agar
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MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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Aseptic Technique and Streak Plates Purpose The purpose of this exercise is to learn aseptic technique procedures and there importance and also to learn to isolate colonies using the streak plate technique. Introduction Bacteria are inoculated (introduced) and cultured (grown) in the laboratory for test studies to determine their morphology (the shape‚ size‚ arrangement‚ and internal structures) and pathology (ability to cause disease). Inoculation has to be performed without adding other microbes
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same control on both sides of the knob and disinfect each side with different disinfectants. There were intervals of 4 minutes‚ in which we let the bacteria sit‚ and swabbed the bacteria off. Immediately after‚ we inserted the sample on a nutrient agar dish. Finally‚ we incubated the samples for approximately 2
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are structures called ascospores. It is these structures‚ ascospores‚ where genetic variation that arises from crossing over is easily seen (Davidson). The organism Sordaria Fimicola is a good example of this process because it is easy to grow on agar plates and because they are easy to be seen when looked at through a microscope (Davidson). There are three strands of Sordaria Fimicola used in this experiment; all were retrieved from an area known as the Evolution Canyon. The Evolution Canyon has two
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