Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction sites generally
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Beetroot Cells with High temperatures and low pH solutions Friday 15th March 2013 Abstract (50) The aim of this investigation was to study the effects of temperature and a low pH solution on beetroot cells. Introduction (200) The general belief amongst our group members was: an increase in temperature will damage the permeability of the plasma membrane. Also‚ the heat will break away the permeability. If the pH solution is 7 or greater there will be no change but the lower the pH the more proteins
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Should all people convicted of a crime have their DNA fingerprints stored on a database?\ A DNA fingerprint is the same for every cell‚ organ and tissue in an organism. DNA fingerprinting has many uses‚ some of which include providing the evidence needed to solve criminal investigations‚ determining genetic relationships and solving paternity disputes. DNA fingerprinting has many benefits in the use of criminal investigations as it can provide the evidence to solve crimes and current mysteries
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AP BIOLOGY- Mitosis and Meiosis Cell Division Lab Part 1-MITOSIS summary: In this experiment first the stages of an onion cell undergoing mitosis are going to be observed and every stage is going to be detected and drawn on paper. A brief description to what is going on should be attached to the pictures. This is important to understand the basics of cell division which is necessary growth‚repair
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Title: Aim: to observe and find out how much starch grains are distributed within a transverse section of a banana Apparatus: banana‚ iodine solution‚ ruler‚ white tile‚ stopwatch‚ microscope slide‚ coverslip‚ filter paper‚ Light microscope Method: 1) A piece of banana was place on a tile and a section was cut across the piece of banana about 5mm thick. 2) The surface of the banana was covered with iodine solution and left for about one minute. 3) The distribution
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Version 2: Identifying Dominant Plant Species 1- Hypothesis Based on the results‚ the bunchberry plant‚ with a scientific name of Cornus canadensis‚ was most frequently seen in station four‚ the ecotone‚ and second most in station five‚ the forest which is most likely in the lower forest since it has a similar environment to that of station four‚ and third most in station three‚ the bog. It was not as frequently seen in areas of higher elevation such as the old field. It should be noted that
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Biology 102 Lab Report: Ventilation and Oxygen Consumption in Fish Methods: The lab consists of two experiments repeated over a period one hour with fifteen minutes interval‚ in order to determine the effect of weight and temperature on ventilation and oxygen consumption of Carassius auratus‚ commonly known as goldfish. Each experiment was carried out by five groups. Measurement of ventilation was determined by recording operculum movement of the fish. Oxygen consumption was measured using oxygen
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BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
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Nervous System and Sensory Organs • dorsal roots and ventral roots - connect Spinal nerves to the spinal cord • medulla -responsible for many involuntary functions such as heartbeat and breathing‚ primary communication pathway between the spinal cord and the rest of the brain‚ • cerebellum - receives input from multiple sensory receptor types and uses this information in coordination of complex body movements • pons- communication between lower and higher brain regions • midbrain- processes
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1. The overall goal of this lab was to carry out experiments that clearly demonstrate the different ways DNA genetic information‚ specifically transduction and conjugation. The first half of this experiment focused on exploring the mechanisms of transduction. This was done by creating a spot titer plate for phage carrying kanamycin resistance and E. coli. E. coli was then proven to have gained kanamycin resistance throughout transduction as demonstrated by its ability to grow on a medium containing
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