Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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scene by comparing with the DNA samples. Polymerase Chain Reaction(PCR) is used to amplify the small amount of Deoxyribonucleic Acid (DNA) for forensic or genetic studies‚ which require necessary product and placed in the thermal cycle. Gel electrophoresis is being run in order to analyze and compare the DNA samples at the crime scene with the guilty suspects. Gel electrophoresis is used to separate DNA using an electric current applied to the gel matrix‚ which causes the DNA samples to move towards
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Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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Efficiency is Dependent Upon Plasmid Grant Zarrinmakan‚ Berkley Kruschke‚ Erika Lawrence. Wed/Fri‚ D-Block‚ 12-January-2015 Introduction Transformation is the genetic alteration of a cell resulting from the direct uptake of exogenous DNA from its surroundings. By doing this lab‚ we will answer the essential question: What influences transformation efficiency? Although there are many possible influences‚ our hypothesis is that plasmid has a positive influence. To test this hypothesis‚ we will set up an
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The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚ and the apparatus
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very useful because of its ability to detect a specific DNA sequence from a large amount of DNA ⎯ even from the whole genome. This specific sequence can be found through a combination of two different techniques: Agarose Gel Electrophoresis and Hybridization‚ which is known as Southern Blotting. This technique if performed in three phases: (I) prepare the gel and (II) make the blot‚ (III) hybridize and visualize blot. In phase one‚ the gel is prepared in three steps: (1) chemical digestion‚ (2)
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Determining Allele Frequencies of the PV92 Alu Element using DNA Isolated from Human Cheek Cells and PCR Amplification Background Alu elements are the most abundant repetitive elements in the human genome that have mobilized throughout primate genomes by retrotransposition over the past 65 million years ago from a 5’ to 3’ fusion of the 7SL RNA gene‚ to reach the present number of more than one million copies. Over the last few years‚ several lines of evidence demonstrated that these elements
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Guidelines for writing laboratory reports Laboratory reports should be written according to the format below (failure to do so will result in marks being deducted): Formatting Font Type: Times Roman Font size: 12 Spacing: 1.5‚ justified Pages : 5 (minimum) - 10 (maximum) [pages must be numbered] Title page You are required to use the lab report submission page available on the LMS and are to include these details: lab no.‚ title of experiment‚ students’ names and ID‚ date of experiment as well
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Abstract: The purpose of this lab experiment was to discover genetically modified organisms. We analyzed our results and found that our sample was GMO positive. These results were attained through polymerase chain reaction and agarose gel electrophoresis. We came to the conclusion that the presence of a band in lane 4 confirms that there are indeed genetically modified organisms. Introduction: Overall‚ the main purpose of this experiment was to conduct gel electrophoresis combined with PCR in order
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Enzyme Lab 6 03/13/2013 Report by Mary Jo Anthony I. Introduction II. Materials and Methods III. Results IV. Conclusion and Discussion Introduction Background Information: This lab allowed us to study chemical reactions and how catalysts will affect the rate of these reactions. The reaction we studied is the breakdown of hydrogen peroxide to water and oxygen and it is vital to life. The molecule hydrogen peroxide is a molecule that is toxic
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