expressed ldhA gene was inserted into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E. coli. The ligation gave 15 colonies of recombinant DNA which later gave 6 recombinant plasmids as revealed by gel electrophoresis. 1. Introduction The NADH-dependent lactate dehydrogenase (EDH) is a key enzyme in the fermentative metabolism
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Labset Three Worksheet 1. What is a carbohydrate profile? Why are they used as a diagnostic or identifying tool? (2) Carbohydrate profiles are specific information on the type and amount of carbohydrate that a product contains. It is used to identify and differentiate two closely related species. 2. What are the carbohydrate profiles of the organisms you tested? (2) The yeast carbohydrate profile came out with glucose and fructose positive and Mannitol negative. The staph epidermidis
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Purpose In this lab‚ we used PCR and gel electrophoresis to identify genetically modified food. Introduction A genetically modified organism is an organism whose DNA or genetic makeup has been modified to code for certain desirable traits("Genetically Modified Foods"). Common genetically modified plants include corn and soy‚ and common genetically modified animals are fish. Many genetically modified plants are coded to resist bugs‚ grow faster‚ and produce bigger fruit‚ while most GMO animals
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Sang Kim Enzyme Catalyst Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A‚ B‚ C‚ and D. In activity A‚ the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction‚ to study the characteristics of an enzyme mediated reaction‚ and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H 2O2 present
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Lab #2: Energy in Cell Virtual Lab - Week 3 NAME: ________________________________________ 1. What is the importance of the light and dark reactions in photosynthesis? Photosynthesis works by absorbing light. Our sun gives off energy and the chlorophyll from the plant absorb this energy. The energy is then used to separate water into hydrogen and oxygen and then they combine hydrogen and carbon dioxide to make sugars. 2. What happens to food energy during photosynthesis? During
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H Week 8 Week 8 Lab: Molecular Biology Review Test Submission: Molecular Biology Lab Assessment Review Test Submission: Molecular Biology Lab Assessment Us er Cours e Introduction To Biology Tes t Molecular Biology Lab As s es s m ent Started 8/19/14 10:14 AM Subm itted 8/19/14 10:22 AM Status Com pleted Attem pt Score 27 out of 30 points Tim e Elaps ed 7 m inutes out of 2 hours . Ins tructions
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Pseudomonas taiwanensis – Enrichment‚ Isolation and Identification Microbiology 521 2/10/12 Purpose: The purpose of this experiment was to enrich Pseudomonas bacteria‚ isolating a species of Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive
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DNA fingerprinting is a method that compares the fragments of DNA. DNA fingerprinting was first invented to detect the presence of genetic diseases. Today‚ DNA fingerprinting is used in different ways. DNA is analyzed using a Southern Blot‚ which allows scientists to observe the base pair patterns. DNA fingerprinting can be used in a few different ways. First‚ to find out if the child belongs to a person DNA fingerprinting may be used. When a child is born‚ it inherits the VNTR’s from the father
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Michelle Kim 2012.1.20 Biology Iso-osmolar Concentration of Carrot Cells Lab INTRODUCTION For a more thorough understanding of this lab introduction‚ the concepts of‚ iso osmolar‚ membrane‚ equilibrium‚ and concentration gradient evaluated. Iso osmolar can be known as the point in which the substance of experimentation faces no change despite the amount of solute inside the solvent( because the solvent and solute concentration is equal. This is where the line on a graph would cross on the
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Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing the most amount
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