Cellular Biology Lab – Homework #3 Due to the week of Nov. 10-14th You may use the lab manual‚ pre-lab lectures‚ and credible internet resources‚ however you may not use your cell bio lab classmates as a resource. You will most likely see this material again on the Final and I highly encourage you to work individually and seek help from myself or your TA.Plagiarism will result in an automatic zero. 1. [15pt]In the cell bio lab‚ we use company manufactured gels‚ however you can make you own polyacrylamide
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Abstract For this experiment we tested the effects of different adrenalin concentrations (0.001%; 0.01%; 0.1%) on the daphnia flea. The daphnia was placed on a slide with cover slip in order to examine its heartbeat by using the microscope. Aim In this Lab we will study the effect of different concentrations of adrenalin on the heart rate daphnia‚ the water flea. It is expected to recognize a threshold of the daphnia in its reaction the three different adrenalin concentrations‚ in terms of heart rate
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Biology lab AIM: To test a leaf for starch APPARATUS/MATERIALS : * bunsen burner‚ tripod stand‚ wire gauze(OR 90oC electric water bath OR hot plate) * 250 cm3 beaker * boiling tube * anti- bumping granules * forceps * test tube holder * white tile * leaf to be tested (hibiscus leaves are excellent) * 90% ethanol * iodine/potassium iodide solution PROCEDURE: The video above shows the steps in Testing a Leaf for the Presence of Starch. The Procedure outlined
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procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended in the lysis
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Bonnie Bell Mrs. Legary Biology P.6 21‚ Sept. 2011 Milk Lab Final This investigation consists of testing the reaction between milk‚ food coloring‚ and dishwashing soap. Different kinds of milk were tested‚ depending on the amount of milk fat content. The investigation was based on 2 questions. Lab one’s problem statement‚ using only milk fat and food coloring was: How does food coloring react in different kinds of milk fat? The second lab’s problem statement‚ using milk fat‚ food
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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1. Given the following parental genotypes‚ show all possible gametes that each could form. Circle each gamete. Note: different gamete types are separated by commas in the table below. Genotype Gametes: AA A Aa A‚ a Bb B‚ b bb b cc c AABB AB AAbb Ab Aabb Ab‚ ab AaBb AB‚ Ab‚ aB‚ ab AaBbCc ABC‚ ABc‚ Abc‚ AbC‚ aBc‚ aBC‚ abC‚ abc 2. In summer squash‚ white fruit color (W) is dominant over yellow (w). If a squash plant homozygous for white is crossed with one homozygous for yellow‚ what will be the apearance
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DNA Aim This practical procedure allows you to amplify a 460 basepair fragment of DNA from within the control region of the mitochondrial genome. This can be done using three water baths or‚ if one is available‚ a thermal cycler (PCR machine). After it has been amplified‚ the DNA is run on an electrophoresis gel. Note: This method has been adapted from one developed by the Dolan DNA Learning Center at Cold Spring Harbor Laboratory. More details are available from the DNA Learning Center’s
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Standard Level Biology Design Lab: How do Smarties and M&Ms compare when examined in 5 areas: mass‚ shell solubility‚ volume‚ density‚ and nutrition facts? ‚ Erin MacNeil SL Biology Kathy DeGrasse Halifax Grammar School March 3rd‚ 2013 Section 1.1 Planning (a) * Introduction * Research Question * Hypothesis * Explanation of Hypothesis * Variables Introduction: In this lab‚ M&Ms and Smarties will be compared in five different ways: mass‚ shell solubility
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