This lab must be typed. Title DNA Fingerprinting Purpose Why are we doing this lab Background 1. What are restriction enzymes 2. When added to a DNA sample‚ what do restriction enzymes do 3. What do you call the specific sequence of bases the enzyme is searching for 4. What is a restriction digestion 5. What is the purpose of the water bath 6. The electrophoresis apparatus creates an electrical field with positive and negative poles at the ends of the gel. DNA molecules are negatively charged.
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John Angelo F. Raule Marvin Reyes Mykee Domingo Aaron Santos Ralph Reyes LBYMATB V26B Report Title Of Activity: Yogurt Making Date Performed: October 4‚ 2012 I. Introduction Last October 4‚ the group performed an activity that involved making our own yogurt. The group prepared the materials and followed the procedures to make the said yogurt. In the activity paper that was given‚ it dictated that during the yogurt making process‚ the bacteria underwent fermentation. “Fermentation
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DNA sequencing From Wikipedia‚ the free encyclopedia [pic] The term DNA sequencing refers to sequencing methods for determining the order of the nucleotide bases—adenine‚ guanine‚ cytosine‚ and thymine—in a molecule of DNA. Knowledge of DNA sequences has become indispensable for basic biological research‚ other research branches utilizing DNA sequencing‚ and in numerous applied fields such as diagnostic‚ biotechnology‚ forensic biology and biologicalsystematics. The advent of DNA sequencing
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DNA FINGERPRINTING Background Reading - Nelson Biology and Campbell Biology Purpose - To understand the basics of DNA fingerprinting used in the Canadian courts for crime convictions and paternity suits. Introduction The process of DNA fingerprinting was developed by Professor Alec Jeffreys at Leicester University in 1984 as a form of genetic analysis. It was first used in the law courts of England in 1987 to convict a man in a rape case. It has now been used successfully in many crime and paternity
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Background on Genomic DNA Isolation and Purification Generally‚ all methods involve the disruption and lysis of cells. This is followed sometimes by the removal of RNA (by RNAses‚ salt or other methods). Choosing which method to use will depend on many selection factors including: DNA is isolated from proteins by several methods including digestion of proteins by the enzyme proteinase K. Proteins are removed subsequently by salting-out‚ organic extraction‚ or binding of the DNA to a solid-phase support
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Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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Enzyme Lab Introduction/ Abstract An enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. They are mainly made up of proteins and can tremendously speed up reactions. E. coli ( a bacterium) has about 1‚000 different types of enzymes floating around in its cytoplasm at any given time. Enzymes can be used to join and even break up molecules as shown in the diagram below. (1)
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Marissa Arias Senior Rhetoric Graf-8 April 14‚ 2014 Gel II Manicure Many women and even some men love to get their nails done‚ but hate how much time it takes to dry and on top of that they are extremely easy to ruin and chip. There is a new type of manicure trending know as Shellac‚ Gel nails‚ or No chip. According to my cosmetology instructor Samantha Rudman‚ this process takes about 35 to 40 minutes but dries in seconds. Many individuals would love to know this process even the men that
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Natalia Rey 080927440 BI338 Lab 1 Measuring the Success of Pisum sativum leaf cell fractionation: SDS-Polyacrylamide Gel Electrophoresis and Western Blot Analysis Introduction In order to evaluate the success of cellular fractionation‚ there are various methods that can be implemented that target specific proteins and/or receptors specific to particular organelles. SDS-PAGE (sodium dedocyl sulfate- polyachrylamide gel electrophoresis) is a technique used to separate proteins based on
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Name: Chase Golden-Froelich Lab #2 Lab Partner: Bailey Martinez Tuesdays‚ Margee Will‚ 11:30 Hypothesis If a predator is restricted in forging range and has a limited food supply then the predator that is not limited will have a higher survivorship than that of the predator that is restricted. a. H0: If a predator is restricted in forging range and has limited food supply‚ there will be no difference in survivorship as a predator who is not restricted. HA1: If a predator is restricted
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