Margaret E. Vorndam‚ M.S. Version 42-0038-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable
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Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another‚ through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith‚ a British microbiologist. Griffith observed that the mutant form‚ non-virulent form‚ of the bacteria Streptococcus Pnumoniae could be transformed into the normal‚ virulent form‚ when injected into mice along with heat killed normal forms. He concluded that somehow
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Lab Report Part II Purpose: To be familiarized with the science and techniques used to identify different types of bacteria based on their DNA sequences. Background Information: The process begins with preparing a sample. Successful identification starts with using a sample that is considered to be good. The first step is to pick up a single colony and drop it into a microcentrifuge tube. A buffer is used to dissolve the cell wall in order to extract bacterial DNA. This step may take several hours
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Materials and Methods Growing the G Strain and Preparing the GCE (rGFP Crude Extract): To grow the bacterial culture‚ use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5‚ which means that time equals zero. At time zero‚ 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded
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2. Steps for Southern Blotting Digest the DNA with an appropriate restriction enzyme. Run the digest on an agarose gel. Denature the DNA‚ which would separate double-stranded DNA into single-stranded DNA. Transfer the denatured DNA to the membrane. Probe the membrane. Visualize your radioactively labeled target sequence. If you used a radiolabeled 32P probe‚ then you would visualize by autoradiograph. 3. a) She placed the nucleotide as she did because the EcoR I cuts in one
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Summary for Quiz Monday‚ May 30‚ 2005 12:13 AM Title Identification of Some Macromolecules Gist of Experiment • Use different tests to check for the existence of macromolecules in various substances o Iodine test checks for starch and/or glycogen o Benedict’s test checks for reducing sugars o Biuret test checks for protein Notes on Underlying Theory Introduction • The most abundant elements in living material are: o Carbon
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KOLEJ MATRIKULASI MELAKA 78300 MASJID TANAH‚ MELAKA Guidelines for Biology Experiments 1.0 Attendance 1.1 Attendance to practical sessions is COMPULSORY. 1.2 If you cannot come to the class due to ill health or emergency excuses‚ please inform the lecturer EARLIER‚ so that another practical session can be arranged for you ON THE SAME WEEK. 2.0 Lab coat 2.1 Wearing lab coat is COMPULSORY. 2.2 Put on the lab coat throughout the class. 3.0 Jotter 3.1 Jotters should contain the summary on the PROCEDURES
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September 23‚ 2012 Introduction: Bacteria are everywhere. Some can be seen with the naked eye and some require a microscope but how do we distinguish one kind of bacteria from another? To answer this question‚ we were required to complete three bacterial labs that helped us to understand what microorganisms are and how to identify and classify them. Thus‚ the main purpose of this project is to identify our unknown microorganisms‚ more specifically‚ our unknown bacteria. There are many ways to distinguish
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Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
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Now You See It – Copper Cycle Lab The purpose of the lab is to discover what happens when someone executes a series of procedures‚ beginning with copper metal. What is done | What is observed | 1. Started with copper‚ Cu (s). | reddish‚ brownish‚ orange-ish‚ powder-like | 2. Added nitric acid‚ HNO3 (aq). | acid turns blue and smells like chlorine. | 3. Added water‚ H2O (l). | stayed the same | 4. Added sodium hydroxide‚ NaOH (aq). | changed consistency‚ gel-like | 5. Heated the mixture
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