Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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chromosomes during the anaphase is known as the splitting apart of the centromere of the chromatids splitting to create a single chromosome which possess a single DNA molecule. 3. What is the term for the DNA form in a nucleus when a cell isn’t undergoing mitosis (ie when a cell is in interphase)? The term for the DNA form in a nucleus when a cell isn’t undergoing mitosis is known as chromatin. 4. Why is mitosis important
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Meiosis and Genetic Diversity in Sordaria 979554296 Biology 110 Lab Introduction: In Israel there exists multiple spots in the mountains called Evolution Canyons‚ which are all located between a southern facing slope (SFS) and a northern facing slope (NFS). What’s particularly interesting about these locations is that despite the two slopes being on opposite sides of a small canyon‚ they exhibit extremely contrasting conditions. The SFS receives multiple times the UV radiation from the sun
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Introduction! ! The Michelson Interferometer is commonly used to determine the wavelength of light or measure very small distances. It was invented by Albert Abraham Michelson and is commonly used in optical interferometry‚ a branch of physics involving a family of techniques one could use to extract information about waves by superimposing them. ! ! The original application of the Michelson Interferometer was to the famous Michelson-Morley experiment in 1887. Prior to Einstein’s
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Formal Scientific Lab Report Osmosis Katy Hunter 10-26-2012 Abstract: The objectives of this lab was to be able to create models of cells with the dialysis tubing to show us how the plasma membrane is selectively permeable‚ to study the effects of osmosis on a model cell‚ and to foresee the effect of solute concentration on osmosis. In order to achieve these objectives‚ we had to fill the dialysis tubing with either water‚ or different amounts of sucrose. We then tied off the tubes and put
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TITLE OF EXPERIMENT 2 : DILUTION 2.1: OBJECTIVES Upon completion of this experiment‚ students should be able to: 1. determine the concentration of coloured solution such as FeCl3 by using dilution and colour differentiating (colorimetric) techniques. 2.2: INTRODUCTION Concentration can be expressed in many different ways such as percentage volume and percentage weight etc. In laboratory‚ normally concentrations were expressed as molarity and normality. Molarity is the
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THE EFFECT OF CHANGE IN pH ON THE ACTIVITY OF ENZYMES Introduction: Enzymes are catalysts that speed up‚ and control chemical reactions within a cell and break down complex molecules into smaller ones. Some of the chemical reactions that take place in the body produce toxic by-products which need to be converted. The liver produces hydrogen peroxide‚ which is extremely poisonous. When the catalase comes in contact with the hydrogen peroxide‚ it is broken down into water and oxygen. Aim:
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Abstract: Bacteria consists of a large domain of prokaryotic microorganism in which can fight of antibiotics allowing the bacteria to become antibiotic resistant. In this lab report‚ we discover the most effective disinfectant that would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly
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In this particular situation we didn’t add enough PGLO into the DNA so ours didn’t glow. In the control lab a different outcomes was observed in each of the four plates. In the LB/amp/arabinose agarose plate containing the +pGLO sample‚ fluorescent green colonies developed. This is because the gene which codes for the fluorescent protein‚ GFP‚ is located near the beta lactamase gene on the pGLO plasmid‚ which protects bacteria from the antibiotic ampicillin. When the cell produced beta lactamase
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OLD COINS TURN TO GOLD DESIGN: Problem or Research Question: How does zinc effects the color change in a copper penny? Hypothesis: If copper and zinc comes together‚ then it will form brass‚ which gives gold color to copper penny. Variables: There were no variables at this experiment PROCEDURES: Materials: Zinc (SN) filling‚ 3M NaOH solution‚ Copper penny‚ tongs‚ Hot plate‚ 100 ml beaker‚ 250 ml beaker‚ Bunsen burner‚ Water‚ Spoon. Procedure: First‚ we turned on the hot plate.
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