Discussion Although only the extraction of strawberry DNA was performed in this lab‚ this section will address the roles of each step taken and the reagents used during the extraction of DNA from animal tissue as well‚ and compare it to the steps taken in the strawberry protocol. As described in the procedure using strawberries‚ the first step was to mash them into pulp using a mortar and pestle. The main goal from this physical disruption was to break the solid material consisting of any connective
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The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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Tan 1 DNA EXTRACTION Aim : To extract the DNA from an egg yolk using various enzymes and to compare with other groups the most effective way to extract DNA. Hypothesis : To be able to observe white springy substances after mixing with enzyme and alcohol. Apparatus : -Test tube‚ spatula‚ glass rod‚ dropper‚ beaker‚ test tube rack‚ skewer. Materials : - 1 egg‚ meat tenderizer‚ salt‚ water ‚ soap‚ isopropyl alcohol 91%‚ pineapple juice. Variables : Manipulated Variable : Responding
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Destiny Thomas Biology October 19th 2012 Period: 3rd Destiny Thomas October 19th 2012 Biology Period: 3rd Introduction In our experiment we used only the red colored life savers. They all had about the same mass. We used 9 life savers. In our experiment we had to dissolve our life savers. The concept of dissolving is to place the
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Gel electrophoresis is a procedure which sorts molecules based on size and charge. The gel in gel electrophoresis refers to the object that separates the molecules. Electrophoreses refers to the force that is used to move the molecules through the gel. There are 2 stages to gel electrophoresis‚ separation and visualization. During separation the gel matrix is placed in an electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel
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tubes 2) Boiling water bath 3) Microscope 4) Slides and cover slips 5) Pasteur pipettes MATERIALS: 1) pH test strips 2) Benedict’s reagent PROCEDURES: Collect the urine samples in a container. Make sure to not spill anywhere in the lab. Complete the tests and all results/ observation are in a proper table. (A) Observation of urine 1) The urine is sniffed and the description of the urine is described. Terminologies like slightly nutty‚ sweet/ fruity or pungent can be used
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Shalacia Gilmore Fall 2012 BIO 1107 Natural Selection Lab INTRODUCTION In the 1850s‚ two scientists by the name of Charles Darwin and Alfred Russell Wallace composed the theory of evolution by natural selection. (1) Darwin characterized several claims needed for natural selection to happen‚ including heritable variation within the population‚ and the presence of more individuals than the environment can support. They also discovered that certain environments favored certain traits. These
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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for the DNA ligation‚ in which the gene products with sticky ends will be inserted to a plasmid vector. Also‚ to transform E.coli DH5α cells by introducing the plasmids DNA which contains the gene of interests into the E.coli strain(DH5α). The plasmid DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein that is resistance to kanamycin‚ this allows for the selection of successful transformed cells. 2. Overview of experiments DNA purification
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DNA replication mechanism is an integral and the most important part of cell division. It is a complicated mechanism that requires a lot of steps and different enzymes. Many scientists were trying to understand the mechanism of DNA replication and its role in the transfer of the hereditary information to the offsprings. DNA replication begins with a single origin of replication that starts off the process by creating a replication bubble. In many eukaryotic cells‚ the DNA strand will have more than
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