Gel electrophoresis is a routine laboratory procedure in biochemical studies that takes advantage of a protein’s amphoteric nature to determine its molecular weight and charge by running the sample through a gel matrix under the influence of an electrically charged field. A popular example of gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide electrophoresis or SDS- PAGE which has been used in this experiment to supposedly determine albumin and casein’s molecular weights respectively. The
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Extracting DNA from Wheat Germ Cells Criteria to be assessed CE Introduction: DNA is the abbreviation for deoxyribonucleic acid. DNA is found in the nucleus of every cell & it stores the information that makes up living organisms. It is a double helix of 4 nucleotides. It contains a code that allows the body to make up proteins. The nucleotides are Cytosine‚ Guanine‚ Adenine and Thymine. (1) Wheat germ comes from wheat seeds. The ’ germ ’ is the embryo‚ which is the part of
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Name: Mugabe Rodrigue Class: Grade 11B Subject: Biology Topic: DNA Extraction From Onion Date: 20/1/2014 Introduction: DNA‚ or deoxyribonucleic acid‚ is the hereditary material in organisms. Nearly every cell in an organism has the same DNA. Most DNA is located in the cell nucleus.The information in DNA is stored as a code made up of four chemical bases: adenine (A)‚ guanine (G)‚ cytosine (C)‚ and thymine (T). The order‚ or sequence‚ of these bases determines the information available for
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Misael Acosta Photosynthesis Lab Report Ms. Rocha AP Biology 4th Period II. Intro/Background 1. In order for oxygen to be present photosynthesis must occur. Photosynthesis takes in light and CO2‚ and through a series of steps produces oxygen. Photosynthesis can be measured by either the disappearance of substrate or the accumulation of product. Examples include the production of O2 or the consumption of CO2. All the oxygen within the leaf disks will be removed before being placed
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Osmosis Lab Report by Evan Gerber Claire Cambron First Lab Report Wednesday 10:30am February 20‚ 2013 Theresa Gburek Abstract The major objective of the experiment was to test the effect of the concentration gradient on the diffusion rate. It was hypothesized that the greater the stronger the concentration gradient‚ the faster the rate of diffusion would be. To test this‚ dialysis tubes were submerged in different concentration fructose solutions. We weighed the tubes at specific
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General Biology I‚ Fall 2011 Lab Report 3 Name: Luan Nguyen Date: 11-30-11 Lab: Gel Electrophoresis Purpose of this lab: In this laboratory investigation‚ students will analyze hypothetical human DNA using restriction endonucleases and gel electrophoresis to match samples from a crime scene to a suspect. Introduction: Your analysis unit will use gel electrophoresis to determine the guilty party. Electrophoresis is a technology in science that allows an individual to separate
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Module 2 Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form
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extract DNA from a strawberry using limited household items. This was accomplished with the prior knowledge that the DNA is stored in the nucleus of the cell‚ and that is an extraction buffer would be needed to acquire the DNA. This experiment had no control group‚ however‚ if there was a control group it might be a group with no extraction buffer. The independent variable in this experiment is the extraction buffer. The dependent variable in this experiment is the ability to extract DNA. The constants
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I. Parts of a Lab Report 1. Introduction: a. Title b. Research Question c. Hypothesis d. Variables e. Control of Variables 2. Materials & Methods a. Materials b. Method 3. Data a. Data b. raw data c. uncertainty d. presentation e. processing data f. Graphs 4. Results/Conclusion a. Conclusion 5. Discussion a. Evaluation 6. References II. Other Help errors and uncertainty A. Design [pic] I. Research Question
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References: Alberte‚ J.‚ Pitzer‚ T.‚ & Calero‚ K. (2012).General Biology I lab Manual. (2nded.) pp 49-61 Boivin M‚ Flourie B‚ Rizza RA et.al. (2002).Gastrointestinal and metabolic effects of amylase inhibition in diabetics. Gastroenterology. Minnesota Copeland‚ Robert Fried‚ G. H.‚ & Handemenos‚ G. I. (2003). Biochemestry.Schaum ’s easy outlines. New York: McGraw-Hill. Fried‚ G. H.‚ & Handemenos‚ G. I. (2001). Biology. Schaum ’s easy outlines. (pp. 119-120). New York: McGraw-Hill. Ibarra
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