Introduction The purpose of this lab was to demonstrate the use of gene therapy on diseases that are caused by a single gene defect. This procedure was demonstrated on two different strains of baker’s yeast‚ EAY 235 and EAY 431‚ which both contained mutations in the LEU2 and TRP1 genes. Neither of these strains will grow without a proper medium that would supply both of these essential amino acids. The EAY 431 strain of yeast also contained a Rad 52 deletion‚ which caused EAY 431 to be a deficient
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Hypothesis: If copper and zinc comes together‚ then it will form brass‚ which gives gold color to copper penny. Variables: There were no variables at this experiment PROCEDURES: Materials: Zinc (SN) filling‚ 3M NaOH solution‚ Copper penny‚ tongs‚ Hot plate‚ 100 ml beaker‚ 250 ml beaker‚ Bunsen burner‚ Water‚ Spoon. Procedure: First‚ we turned on the hot plate. Then‚ we filled 250 ml beaker with 125 ml of water. We put half a spoon of zinc fillings. We add 20 ml of 3M NaOH solutions in it
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Nervous System lab was designed to measure reaction times by using a variety of tests that affect the heart rate. These tests were the startle‚ stroop‚ Valsalva maneuver‚ diving reflex‚ and a test of our choice. The test our group chose to measure the heart rate in response to doing a trust fall to anticipate the stress right before the fall. To begin this lab we chose a participant‚ and then hooked their finger up to the pulse transducer sensor. Using the chart 5 program on the lab computer the participant’s
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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inserted into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E. coli. The ligation gave 15 colonies of recombinant DNA which later gave 6 recombinant plasmids as revealed by gel electrophoresis. 1. Introduction The NADH-dependent lactate dehydrogenase (EDH) is a key enzyme in the fermentative metabolism
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Bubble Inside a Bubble Materials • • • • • • • • Granulated sugar (we had our best results using Imperial Sugar and Dixie Crystals) Dish soap Water Tablespoon Scissors Pipette Cup Adult supervision Bubbles form because of a combination of water’s hydrogen bonds and the oily film you can see shimmer in the light. The oily film you see is actually two separate layers of soap attached to‚ and surrounding‚ hydrogen-bonded water. Solar Oven S’mores Materials • Pizza box • Two clear sheet protectors
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2.1. Test article Carmoisine also known as E122 or Food Red 3‚ Brillant carmoisine O‚ Azorubin S‚ Acid Red 14‚ CAS No. 3567-69-9 or C.I. 14720‚ is a di sodium salt of disulfonates of 2-(2 quinolyl) – 1‚ 3 indandione and is obtained as red to maroon colour powder (Fig. 1). Dye content in the substance is 88%‚ sodium chloride/sulphate is less than 12%‚ water insoluble matter less than 0.2%‚ Arsenic less than 1 ppm‚ lead less than 0.01 ppm‚ heavy metals less than 40 ppm. Carmoisine was certified by
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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Pre-lab questions: 1. Algae belong to what Kingdom? They belong to the Kingdom Protista. 2. What are the three types of multicellular algae? The three types if multicellular algae are chlorophyta which are green algae‚ phaeophyta which are brown algae‚ and rhodophyta which are red algae. 3. Are algae autotrophs or heterotrophs? Algae are autotrophs. 4. What organelle allows plants (and plantlike protists) to photosynthesize? The chloroplasts allow plants and plantlike protists to photosynthesize
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Formal Scientific Lab Report Osmosis Katy Hunter 10-26-2012 Abstract: The objectives of this lab was to be able to create models of cells with the dialysis tubing to show us how the plasma membrane is selectively permeable‚ to study the effects of osmosis on a model cell‚ and to foresee the effect of solute concentration on osmosis. In order to achieve these objectives‚ we had to fill the dialysis tubing with either water‚ or different amounts of sucrose. We then tied off the tubes and put
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