Function 16 October 2014 Gel Filtration and Electrophoresis Objective The essential goal of the experiment was to separate proteins in a solution based on size in different fractions. The relative protein content for each one fraction was found through the utilization of an amido black-based protein assay. Later in the trial polyacrylamide gel electrophoresis was utilized to separate BSA from hemoglobin. Methods I. Gel Filtration and Protein Assay: 1. A slurry of Bio-Gel P-100 beads in water was
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quantities present in the urine. APPARATUS : 1) Test tubes 2) Boiling water bath 3) Microscope 4) Slides and cover slips 5) Pasteur pipettes MATERIALS: 1) pH test strips 2) Benedict’s reagent PROCEDURES: Collect the urine samples in a container. Make sure to not spill anywhere in the lab. Complete the tests and all results/ observation are in a proper table. (A) Observation of urine 1) The urine is sniffed and the description of the urine is described. Terminologies like slightly
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Shalacia Gilmore Fall 2012 BIO 1107 Natural Selection Lab INTRODUCTION In the 1850s‚ two scientists by the name of Charles Darwin and Alfred Russell Wallace composed the theory of evolution by natural selection. (1) Darwin characterized several claims needed for natural selection to happen‚ including heritable variation within the population‚ and the presence of more individuals than the environment can support. They also discovered that certain environments favored certain traits. These
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EXPERIMENT REPORT (PREPARATION OF CdS) Experiment Purposes 1. To master the proper operations as well as the suitable situations of atmospheric pressure filtration‚ vacuum filtration‚ centrifugal separation & precipitation washing 2. To understand the relation between precipitation state % precipitating conditions 3. To learn the way to examine whether reactants are fully precipitated 4. To learn the principals of powder material synthesizing through room temperature solid phase method
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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water instead of the sample should be included. Independent: different types of food samples Dependent: different types of food substance presence in food sample Assumption: Controlled: using the same amount of food sample in different test Procedure: Solid: Grind the food with distilled water. Squeeze the ground materials through layers of muslin. Liquid: Put 1 cm3 of the liquid food sample into test tubes. -grease spot test: A drop of liquid food sample was added to a filter paper
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Nick Sarris‚ April 3‚ 2013‚ D-Bell Biology Virtual Electrophoresis Lab – Genetic Science Learning Center Use the link to complete the following lab. Submit through edline when you are finished http://learn.genetics.utah.edu/units/biotech/gel/ Title‚ name‚ date and bell (8 pts) Place your answer below the question and skip between questions (2 pts) Each question is worth 3 points 1. Why can’t DNA be sorted physically‚ using a microscope?- They are so tiny that they are unable to be
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® Welding Procedure Guide An easy to follow guide covering the preparation of welding procedure data sheets © Copyright 2008 CWB Group - Industry Services ® © Copyright CWB Group - Industry Services Revised September 2008 All rights reserved. CWB Group 1-800-844-6790 www.cwbgroup.org ® TABLE OF CONTENTS 1.0 Introduction 2 2.0 Welding Engineering Standards 3 3.0 Welding Procedure Specification (WPS) 3 4.0 Welding Procedure Data Sheet
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determine the boiling point for the known and unknown. Then use a graph to calculate the slope and then use Clasius- Clapeyron equation to find the heat vaporization. Procedure: Refer to pages 155-167 of Chemistry 1210 General Chemistry Laboratory Manual Hayden- McNeil Publishing Inc. 2012-2013 Report Sheet: See attached report sheet and graphs. Sample Calculations: 1/t= 35 C +273 K = 1/308 =0.0325 1/K Ps= Pb (1- Vr/V * T/Tr)= 746(1-5/8*308.15/343.15)=325.3
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Name: Siti Nur Amalina binti Mohamad Sukri Class: M12E Title: Cell Respiration and Aquatic Animal Aim: To investigate the effect of a change in the temperature of water on the respiration rate of a goldfish. Research question: Does a change in temperature of water affect the respiration rate of a goldfish? Introduction: “The goldfish is a domesticated subspecies of a carp like fish called Carassius auratus. Goldfish are usually kept as pets‚ feeding on fish food‚ algae‚ plankton and other
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