The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
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Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML --) . Ni2+ agarose affinity chromatography was used to purify the crude
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Biology 101 Popcorn experiment Lab partners Robert Barham Michelle hall Diva Mancada Kelley Pritt 1 Introduction Popcorn‚ or popping corn‚ is a type of corn which explodes from a kernel and puffs up when heated. It is a popular snack food‚ especially in movie theaters. Popcorn can be salted or sweetened. Air popped popcorn is naturally high in fiber‚ low in calories and fat‚ contains no sodium and is sugar free. This can make it an attractive snack for people with caloric or
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The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚ and the apparatus
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Fifty milliliters of 1.5% agarose solution was swirled and inserted into the microwave for 1 to 2 minutes until the agarose solution was clear. The solution was put into a cooling bath of 60 °C for 15 minutes. After cooled‚ the agarose was poured into the prepared gel-casting tray for 30 minutes until it became solid. The well-forming comb was then removed and 1X TBE buffer was added to fill in
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The Microscope Lab Brandy Talley PRCC Biology Lab Online June 13‚ 2013 Purpose: The purpose of this lab is to learn about different types of microscopes that we use today‚ learn the parts of the microscope‚ and how to properly use a microscope by exploring various slides. Materials: *Microscope *Letter “e” slide *Onion root slide *Bacterial capsule slide *Cheek smear slide Lab Notes: 1.What is the difference between a compound
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Meal Worm Cellular Respiration Experiment Introduction Cellular respiration provides energy to all living organisms by collecting energy from food molecules. The cell then rearranges complex molecules to simpler molecules and uses that energy to make a molecule that stores energy called adenosine triphosphate also known as ATP. Cellular respiration can occur with or without oxygen. When it occurs without oxygen‚ it is called anaerobic respiration. There are very few organisms that can get
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Pixar Facts 1.) In order to give Dash a realistic out-of-breath voice in "The Incredibles‚" director Brad Bird made actor Spencer Fox run laps around the studio.4 2.) Every Friday during the production of “Brave‚” each Pixar employee was told to wear a kilt for the fun of it. 3.) Shortly after completing Toy Story‚ the Pixar chief creative staff of John Lasseter‚ Peter Docter‚ Joe Ranft and Andrew Stanton got together for a lunch meeting at the Hidden City Café in 1994 that would become one of
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Chapter 12 (Part 2) Explain the principle of independent assortment and its relation to meiosis: The segregation of chromosomes in anaphase I of meiosis explains Mendel’s observation that each parent gives one allele for each trait at random to each offspring‚ regardless of whether the allele is expressed. The segregation of chromosomes at random during anaphase I explains Mendel’s observation that factors‚ or genes‚ for different traits are inherited independently of each other. Apply the
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Part 2: Safe work with large inductances 1. One of the main hazards of using inductances is electrical energy stored in the magnetic field. If a circuit containing an inductance is interrupted then the stored energy can release through air (forming an arc). This can destroy a switch contact‚ burn fingers‚ create other damage. In the circuit of Figure 1‚ show how to mitigate the risk (pay attention to polarity!). We give the elements in this circuit some simple values for the purpose of
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