The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
Premium DNA Gene Molecular biology
Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML --) . Ni2+ agarose affinity chromatography was used to purify the crude
Premium DNA Bacteria Escherichia coli
The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚ and the apparatus
Premium Gel electrophoresis Molecular biology DNA
Biology 101 Popcorn experiment Lab partners Robert Barham Michelle hall Diva Mancada Kelley Pritt 1 Introduction Popcorn‚ or popping corn‚ is a type of corn which explodes from a kernel and puffs up when heated. It is a popular snack food‚ especially in movie theaters. Popcorn can be salted or sweetened. Air popped popcorn is naturally high in fiber‚ low in calories and fat‚ contains no sodium and is sugar free. This can make it an attractive snack for people with caloric or
Premium Popcorn
Fifty milliliters of 1.5% agarose solution was swirled and inserted into the microwave for 1 to 2 minutes until the agarose solution was clear. The solution was put into a cooling bath of 60 °C for 15 minutes. After cooled‚ the agarose was poured into the prepared gel-casting tray for 30 minutes until it became solid. The well-forming comb was then removed and 1X TBE buffer was added to fill in
Premium DNA Gene Genetics
The Microscope Lab Brandy Talley PRCC Biology Lab Online June 13‚ 2013 Purpose: The purpose of this lab is to learn about different types of microscopes that we use today‚ learn the parts of the microscope‚ and how to properly use a microscope by exploring various slides. Materials: *Microscope *Letter “e” slide *Onion root slide *Bacterial capsule slide *Cheek smear slide Lab Notes: 1.What is the difference between a compound
Premium Lens Microscope Optics
Limiting Reagent and Percent Yield Aim To determine the limiting reagent between the reaction of lead (II) nitrate and potassium iodide. To determine the percent yield of lead (II) iodide. Date Started: 13/4/12. Finished: 19/4/12. Data collection and processing Measurements: * Amount of distilled water: 75.0ml ± 0.5ml. * Mass of watch glass: 31.65g ± 0.01g. * Mass of watch glass + potassium iodide: 32.45g ± 0.01g. * Mass of potassium iodide: 0.8g ± 0.02g. * Mass of watch
Premium Stoichiometry Yield Molecule
Tittle : Investigation of the Enzymatic Effects of Materials on Hydrogen Peroxide Solution Objective: To investigates the enzymatic effect of various materials in the hydrogen peroxide solution. Table 1 Test Tube Contents with 5 cm3 hydrogen peroxide Observations before and after using wood splint Observation of after Observation of after adding hydrogen using wooden glowing peroxide splinter 1 Fresh liver Moderate
Premium Oxygen Hydrogen peroxide Catalase
Chemotherapeutic Agents of Control: Introduction: Chemotherapeutic agents are chemical substances used to treat various forms of infectious diseases. The chemotherapeutic agent works by stopping the bacteria from reproducing. There are two different kinds of chemotherapeutic agents. The first kind is an antibiotic‚ which slow down the growth of microorganisms. The second kind of chemotherapeutic agent is synthetic drugs. Synthetic drugs are artificially made in a laboratory. Chemotherapy
Premium Bacteria
ENG 4UI: ISU Essay Outline ----------------------- Introduction: All life and existence makes an unavoidable journey towards an ultimate end. Immortality is a mythical unattainable existence‚ barred by the presence of entropy. Mortality is a constant found in everything that was‚ is and will be. Hook:
Premium Philip K. Dick Do Androids Dream of Electric Sheep? Blade Runner