Antibacterial action of the irrigants against Enterococcus faecalis in the root canal The overall percentage reduction of E. faecalis inside the root canal after biomechanical procedures was presented in table1. All the irrigants significantly reduced bacteria in comparison with positive control group (P< 0.01). The average reduction of bacteria in the presence of E.galbie did not have any significant difference with 2.5% NaOCl‚ CHX 2%‚ CHX 0.2% and M.communis L. (P > 0.05). The average reduction
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The lipase gene sequence from Alcaligenes sp. JG3 was translated into amino acid sequence using two methods‚ manual translation and ExPASy online software. By manual method‚ amino acid sequence was obtained via codon translation one by one as shown in Figure IV.7. The sequence reference used is the lipase from A. faecalis MOR02 due to having high similarity with the lipase sequence from strain JG3 during CLUSTAL alignment and the alignment of the deduced amino acid with the referred sequence was
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Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus mirabilis‚ Proteus vulgaris‚ Pseudomonas aeroginsoa‚ Alcaligenes faecalis‚ Rhizobium radiobacter‚ Salmonella typi‚ Serratia marcescnes‚ Shigella flexneri‚ or Yersina pseudo tuberculosis. Ten of the unknown bacteria were classified as Gram-positive bacilli: Bacillus cereus‚ Bacillus circulans‚ Bacillus coagulans
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then flooded the plate with Iodine and were able to observe and record starch Hydrolysis. (Table 1) * In order to test carbohydrate catabolism under anaerobic conditions we tested cultures of Pseudomonas aerugenosa‚ Escherichia coli‚ and Alcaligenes faecalis in tubes containing a glucose medium. After inoculating each tube with each of the bacteria 5mm of mineral oil was poured into one of the tubes containing bacteria to observe anaerobic respiration and while one remained without mineral oil
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negative organisms were positive for glucose except for two. Since our organism tested negative for glucose it helped narrow it down to only those two organisms‚ Alcaligenes faecalis and Pseudomonas aeruginosa. The other tests that we performed on our unknown were not really helpful when it came to determining if it was Alcaligenes faecalis or Pseudomonas aeruginosa because they both had all the same characteristics when it came to the lactose‚ mannitol‚ motility‚ indole‚ citrate‚ H2S‚ urease‚ methyl
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Microbiology Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques
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Introduction to Microbiology Laboratory report №10 Physical factors affecting growth microbes: Temperature‚ pH and oxygen requirement. Student: Temirlan Aitbekov Lab partner: Kanat Sadykov Instructor: Alessandra Clementi‚ MD‚GP Lab date: 7/11/14 Due date: 14/11/14 Nazarbayev University Abstract: This experiment is directed
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Screening and identification of rhizobacteria isolated from heavy metal contaminated soils INTRODUCTION As a result of human activities such as mining‚ agriculture and industrial activity‚ heavy metal pollution has become one of the most serious environmental problems today. Elevated levels of heavy metals not only decrease soil microbial activity and crop production‚ but also threaten human health through the food chain. Soil microorganisms can degrade organic contaminants‚ while metals need
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acidophiles (acid-loving)‚ neutrophiles (neutral ph range)‚ or alkaliphiles (alkali-loving). The one that causes disease in humans would be the neutrophiles‚ which have an ideal pH range of 5.4 to 8.0. There are exceptions‚ however‚ like Alcaligenes faecalis and Vibrio choleae‚ which are both alkaliphiles and can infect humans. Bacteria can also be classified as to the temperature range in which it grows best. Psychrophiles are cold-loving and can live in temperatures from 0 20 C. Mesophiles
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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