Isolation‚ Expression and Cloning of Lactate Dehydrogenase Gene from Lactobacillus bulgaricus Lactobacillus bulgaricus is used for industrial production of yogurt mainly because of his fermentative capability. In this experiment‚ the gene encoding the enzyme lactate dehydrogenase [ldhA] from the bactaria was isolated and cloned. The expressed ldhA gene was inserted into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E.
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thousands of proteins. Purification allows scientist to know what molecule substrates are present‚ if the enzyme activity is regulated‚ the size‚ and what subunits are in the protein. This particular experiment focuses on lactate dehydrogenase from a beef heart. Lactate dehydrogenase (LDH) catalyzes the reaction where lactate is oxidized to pyruvate and NAD+ is reduced
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electron transport chain process is for the NADH2 produced during glycolysis‚ the intermediate step‚ and the citric acid cycle to be attracted to Complex I (FMN ·FeS)due to its high affinity for NADH2. This attraction pulls NADH2 to Complex I (NAD dehydrogenase) and the two electrons from H2 are pulled off by the FeS (ferrous sulfate) leaving two H+ ions and NAD+. These molecules repel each other and this results in the NAD+ being recycled. The hydrogens remain in the matrix while the two electrons are
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rise in the number of pensionable-aged Scots by 2035 (Scottish Government‚ 2011b)‚ this increase in chronic disease has led to a focus on the health risks associated with our behaviour (Dixon and Johnston‚ 2010). Risk Factor: Excessive Alcohol Consumption Alcohol has an irritant effect‚ contributing to arteriosclerosis‚ ultimately increasing the risk of hypertension‚ heart disease and stroke (Harrington-Dobinson and Blows‚ 2007a). It irritates the mucous lining of the gastrointestinal tract and
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PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine
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Part of Lactate Dehydrogenase responsibility is to catalyzes the reaction of pyruvate and lactate of NADH AND NAD+. As it converts pyruvate‚ the final product of glycolysis‚ to lactate‚ if oxygen is absent or in minimal amounts‚ it will perform the opposite reaction during another cycle‚ which is the Cori cycle. The Cori cycle takes place in the liver. When we exercise our cells get significant amounts of oxygen and sugar is broken down quickly and very efficiently. On the other hand‚ when we start
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Factors for Alcohol Abuse Michelle Clayton Indiana Institute of Technology Introduction to Psychology/1700 August 2‚ 2012 Rita Polivick Abstract Alcohol abuse is a growing problem in the military‚ and a costly one. The present study evaluates the potential role of psychological hardiness an individual resilience resource‚ to stress-related problem drinking in a military population. We assess the association of psychological hardiness and avoidance coping style with alcohol use patterns
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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effect that developing offspring? Does drug or alcohol abuse actually hurt the unborn child? What about the mental health of the carrying mother; would depression or other mental illnesses hurt the baby? Do eating habits or exercise actually produce different traits once the child is born? During pregnancy‚ ingesting and doing a wide variety of things can cause several various effects on the unborn child of the mother depending on the activity. Alcohol use during pregnancy is widely known for being
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HOW DOES ALCOHOL MAKE YOU DRUNK? Drinking alcohol can be similar to a drug addiction unless the consumption is being monitored and controlled. Once alcohol is absorbed into the bloodstream‚ it quickly moves throughout the body causing changes in mood‚ feelings‚ and behavior. Most people drink in order to be affected by these changes. Depending on the age‚ weight‚ and gender of a person and how much someone may be drinking can determine how fast the feeling of being drunk can begin. People
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