An international journal published by the Nigerian Society for Experimental Biology Printed in Nigeria Cofactor interactions in the activation of tissue non-specific alkaline phosphatase: Synergistic effects of Zn2+ and Mg2+ ions Femi J. OLORUNNIJI*‚ Adedoyin IGUNNU‚ Joseph O. ADEBAYO‚ Rotimi O. ARISE and Sylvia O. MALOMO Department of Biochemistry‚ University of Ilorin‚ P.M.B. 1515 Ilorin‚ Nigeria Received 19 March 2007 MS/No BKM/2007/028‚ © 2007 Nigerian Society for Experimental
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Hypophosphatasia is one of many disorders that resemble oseogenisis imperfecta‚ also known as “Brittle Bone Disease”. A mutation in the gene that codes for tissue-nonspecific alkaline phosphatase‚ or ALP‚ is believed to be the cause. That is the central hostile regulator for bone mineralization. The patient who has Hypophosphatasia receives one defective gene from each parent. (Hypophosphatasia‚ eMedicine) Males‚ females‚ and all races are equally affected. Incidence has been rated
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tests were used to determine significant differences among groups. P < 0.05 was considered to be significant. 6.3 Results 6.3.1 Liver function tests Liver toxicity markers were assayed to assess hepatic injury. The activities of alkaline phosphatase (ALP)‚ acid phosphatase (ACP) and gamma glutamyl transferase (GGT) were significantly altered in the diabetic control group‚ indicating damage to hepatocytes. The hepatic toxicity markers in normal rats treated with ABE did not show any significant difference
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J.L. SOAP NOTE Subjective J.L. is a 63-year-old white female who presented to the ER with right lower extremity pain and swelling with subsequent changes to the skin‚ including formation of bullous ulcers. She had blistering over the past four days that started to enlarge. The doctor prescribed her Bactrim. Erythema‚ swelling‚ and pain progressively got worse. She denies any fever or chills. PMH: COPD‚ Hypertension‚ Diabetes mellitus‚ Osteoporosis‚ Fibromyalgia‚ Osteoarthritis‚ Morbid obesity
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Alkaline Phosphatase was isolated using various techniques. BCIP spot test was performed after the anion exchange chromatography was done and the fractions were collected. The picture of BCIP spot test of the 20 fractions can be seen in figure 1. The intensity of each spot was different from each other. The more intense color the spot was‚ the more concentrate the AP was. The fractions were chosen to combine together based on the intensity‚ which made the stage 4 enzyme. Different volumes in each
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Alkaline soil is referred to by some gardeners as”sweet soil”. The pH level of alkaline soil is above 7‚ and it usually contains a great deal of sodium‚calcium and magnesium. (1) Alkaline soil occurs in areas with high limestone content‚ easy souble phosphorus and calcium binding‚ formation of insouble calcium salts of phosphorus‚ reducing the effectiveness of phosphorus. Also‚ in calcareous soils‚ boron‚ manganese‚ molydenum‚ zinc‚ iron greatly reduces the effectiveness of the crop often feel inadequate
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Biochemistry Unit The Kinetics of Alkali Phosphatase Inhibition 1. OVERVIEW This practical builds on the enzymology lab skills you learned in the Acid Phosphatase practical. Again‚ you will measure the initial reaction velocity (V 0) of an enzyme reaction‚ but this time in the absence and then presence of an inhibitor. Last time you used Acid Phosphatase (Prac 1)‚ but this time you will use the enzyme Alkali Phosphatase. These enzymes have different primary (and hence tertiary) structures
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Division of City Schools City of Mandaluyong Science High School E. Pantaleon St. Hulo‚ Mandaluyong City BLACK MIX FROM ALKALINE BATTERIES AS PIGMENT FOR THE MANUFACTURE OF INKJET PRINTER-FRIENDLY INK Submitted in partial fulfilment of the requirements in Research II By Psalm Israel Rendon Hazel A. Lunas Diane Clarisse Ortega IV-Chlorine SY 2011 -2012 September 2011 TABLE OF CONTENTS TITLE PAGE.........................................................................
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Expt. BL 304 Kinetics of Alkaline Phosphatase Objective To study the kinetics of alkaline phosphatase. Procedure 1. Sodium carbonate - sodium bicarbonate buffer: Dissolve 20 ml of 0.2 M solution of sodium carbonate (2.12 gm in 100 ml distilled water) and 230 ml of 0.2 M sodium bicarbonate (4.12 gm in 250 ml distilled water) to make up the volume 250 ml with pH 9 -9.2 2. 5 N sodium hydroxide solution (10 gm in 50 ml distilled water) 3. Substrate stock solution: Dissolve 0.1 gm of p-nitrophynyl
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