TEXT BOOK OF BIOCHEMISTRY For 2nd year Medical Laboratory Technician Course (INTERMEDIATE VOCATIONAL) Author : S. Srinivasa Rao‚ B.Pharmacy‚ Jr. Lecturer in M.L.T.‚ Alluri Sri Rama Raju Govt. Junior College‚ Shanthi Nagar‚ Khammam. Editor : S. Kamalakar Rao‚ M. Pharmacy Senior Lecturer in Pharmacy‚ Department of Biotechnology‚ Govt. institute for post diploma courses in engineering and technology‚ Ramanthapur‚Hyderabad. INDEX Chapter I. Page No. Instrumental methods of Biochemical
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IMMUNOCHEMICAL TECHNIQUES: Immunochemistry is an advanced area of immunology. It deals with the chemical components and chemistry (chemical reactions) of immunological phenomena that is of antibody and antigen. Immunochemical methods are processes utilizing the highly specific affinity of an antibody for its antigen. It detects the distribution of a given protein or antigen in tissues or cells. The methods used for the immunochemical analysis are called Immunochemical techniques. Characteristics/Advantages
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CHAPTER 13 HOW CELLS OBTAIN ENERGY FROM FOOD 2009 Garland Science Publishing The Breakdown and Utilization of Sugars and Fats 13-1 Glycolysis is an anaerobic process used to catabolize glucose. What does it mean for this process to be anaerobic? (a) No oxygen is required. (b) No oxidation occurs. (c) It takes place in the lysosome. (d) Glucose is broken down by the addition of electrons. 13-2 Which of the following stages in the breakdown of the piece of toast you had for breakfast generates
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Types of Hepatitis Lab test results show a positive anti-HCV and confirm that Chad has Hepatitis C. The nurse explains to Chad that Hepatitis is liver disease with inflammation of the cells of the liver. Chad states "I can’t have Hepatitis. I had all three of the vaccinations before I started working as a paramedic." There are five major types of viral Hepatitis: A‚ B‚ C‚ D‚ and E. 1. What information about the vaccination series for Hepatitis received by healthcare workers will the
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Boone Thomas Personal Impact Paper Nurs/427 Health and Chronic Disease Management 01 October 2011 Personal Impact Paper |Student: |Boone Thomas | |Date of Care: |28 September 2011 | | | | | |Patient | | | |
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BIMM 101: Recombinant DNA Lab Lab 7 & 8 Assignment Part B 1. A genomic library is a “collection of recombinant vectors or clones‚ among which is representative of the entire genome of the organism” (BIMM 101 Lab Manual‚ 47). In order to create a genomic library‚ genomic DNA from Vibrio fischeri was first isolated then treated with Sal I restriction enzyme to generate inserts (smaller fragments of DNA). Sal I restriction enzyme was also used to treat the vector plasmid in order to digest
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small and were not randomized or double-blind‚ however. Reported adverse effects of bitter melon include hypoglycemic coma and convulsions in children‚ reduced fertility in mice‚ a favism-like synrome‚ increases in γ-glutamyltransferase and alkaline phosphatase levels in animals and headaches. Bitter melon may have additive effects when taken with other glucose-lowering agents. Adequately powered‚ randomized‚ placebo-controlled trials are needed to properly assess safety and efficacy before bitter
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3 Yeast Metabolism Metabolism refers to the biochemical assimilation (in anabolic pathways) and dissimilation (in catabolic pathways) of nutrients by a cell. Like in other organisms‚ in yeast these processes are mediated by enzymic reactions‚ and regulation of the underlying pathways have been studied in great detail in yeast. Anabolic pathways include reductive processes leading to the production of new cellular material‚ while catabolic pathways are oxidative processes which remove electrons
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hydrolysis by p-nitrophenylphosphate (PNP) as a substrate by the enzyme phosphatase. Abstract The hydrolysis of p-nitrophenyl phosphate has been studied in human red blood cells. To see if hydrolysis was related to the functioning of the sodium pump. Acid phosphatase catalysis’s the hydrolysis of p-nitophenyl phosphate under four different objectives ph‚ temperature‚ substrate inhibition and a competitive inhibitor. The phosphatase and PNP were placed in test tubes with different concentration of each
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influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown phosphatase analyzed showed it was an acidic phosphatase. Ph 5 had the most enzyme activity and pH 9
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