Natalia Rey 080927440 BI338 Lab 1 Measuring the Success of Pisum sativum leaf cell fractionation: SDS-Polyacrylamide Gel Electrophoresis and Western Blot Analysis Introduction In order to evaluate the success of cellular fractionation‚ there are various methods that can be implemented that target specific proteins and/or receptors specific to particular organelles. SDS-PAGE (sodium dedocyl sulfate- polyachrylamide gel electrophoresis) is a technique used to separate proteins based on
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Osteo - comes from the ancient Greek word for bone‚ sarcoma – is the name given to cancer. Osteosarcoma is the most common type of primary bone cancer. It starts when bone cells become abnormal‚ growing out of control to form a lump of cancerous tissue. The cancerous tissue consists of abnormal cells that are attempting to make bone. Being able to see this abnormal bone under a microscope helps to confirm a diagnosis of osteosarcoma. The cause really is unknown but genetics can play a role. The majority
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ETHNOMEDICAL SURVEY This survey was conducted in a Malay village name kampong Tanjung Sabtu located at West Kuala Terengganu‚ the capital city of Terengganu in Peninsular Malaysia. Most the villagers are farmers and some government staffs. This data obtain from the conversation adult villager‚ local healer and herbalist by using the ethnomedical enquiries. Mostly the villagers willing to share their knowledge on the uses of some medicinal plants. Total 52 plants were recorded to be used among
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The purpose of the first week experiment was to determine the effect of Nitrophenyl phosphate concentration‚ the substrate‚ on the enzyme‚ acid phosphatase‚ reaction. In an enzyme reaction‚ there is substrate that binds to the active site of the enzyme and product is the end result. The enzyme is the catalyst for the reaction which means that it speeds up the process. Without enzymes‚ the majority of reactions would not occur because the activation energy level would be unattainable. With this in
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derived from the Latin phrase “in yeast”‚ is an organic catalyst‚ most often proteins‚ that accelerate specific chemical reactions by lowering the required activation energy necessary for that reaction to occur. One enzyme in particular‚ Acid Phosphatase‚ is a critical enzyme in our every day biological functions. Phosphorus plays a vital role in energy transfer and metabolic regulation and is an important component of phospholipids and nucleic acids. (Hiroshi‚ 2001‚ Cavigis‚ 2001) Furthermore
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electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚ and the apparatus will be connected to a constant voltage source (175 V) for
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Lafora progressive myoclonus epilepsy is a fatal form of autosomal recessive neurodegenerative disorder that is characterized by the presence of insoluble polyglucosan inclusion bodies‚ known as Lafora bodies. They are generally found in many of the body’s tissues such as within the cytoplasm of the cells of the heart‚ liver‚ muscle‚ and skin but the signs and symptoms of the disease are limited to the nervous system. The disease presents with epileptic seizures followed by progressive central nervous
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About Chinese medicine (Pien Tze Huang) In 1555‚ during the Ming dynasty imperial Zong Shi‚ a royal physician fled to the city of Zhangzhou (Fujian province) because it is not satisfied with the emperor was very cruel. The physician was settled in a temple located in the East Pushan under Zhangzhou City as a monk. He witnessed the suffering and misery of the people due to incurable disease so that he felt compelled to provide aid to all residents. By using a secret potion from the palace‚ he finally
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EGD ERCP Esophageal Motility Studies ENDOSCOPIC TESTS (cont’d) Colonoscopy Proctoscopy Sigmoidoscopy Laboratory Tests Gastric Analysis CBC PT (prothrombin time) INR PTT (partial thromboplastin time) Bilirubin Blood proteins Alkaline Phosphatase LDH GGT AST ALT Cholesterol & Triglycerides Amylase CEA Nursing Assessment Chief complaint Past Medical history Family history Review of systems/PE Evaluation of diagnostic data Abnormal Assessment Findings Distention Firmness
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The result of this study showed that bee bread extract has a protective effect in aluminum induced anemia and it significantly ameliorates hepato-renal toxicity and reduces the elevated CRP level and monocytes and leukocytes counts encountered after aluminum exposure. Aluminum alone caused significant anemia‚ elevation of BUN‚ CRP‚ leukocytes and monocytes counts and liver enzymes‚ ALT and AST‚ and deceased creatinine clearance. Chemical analysis and antioxidant study showed that it contains fat
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