performed in order to analyze the effects of pH‚ enzyme concentration‚ and temperature on the catalytic rate on enzyme ALP. The initial experiment was done to approximate the optimum pH for the activity of the enzyme. The hypothesis was that alkaline phosphatase activity will be critically affected by pH effects. The hypothesis was valid because the data shows that the pH values for the acidic‚ basic‚ and neutral tubes increased over time‚ absorbing at similar rates. The basic tube had the highest
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All glassware that was being used had to be cleaned so that other old substances did not interfere with the outcome of the results. This experiment included an unknown phosphatase buffer solution and enzymes A and B. The experiment had two sections. In Part A‚ a colorimetric assay was conducted by using a spectrophotometer. The substance was a clear‚ colorless liquid that turned yellow. This reaction occurred because p-nitrophenyl
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epigastrium‚ and right upper quadrant. No other mass‚ hepatosplenomegaly‚ or hernias noted. Pelvic and rectal‚ unremarkable. DIAGNOSTIC DATA: White blood cell count 10.1 with 79% segs. PTT is normal. SMA shows elevated bilirubin at 1.54 with alkaline phosphatase at 442. Sonography reveals multiple gallstones with a stone in the common bile duct. These were reviewed by Dr. Singh‚ radiologist. Chest x-ray was unremarkable. IMPRESSION: Cholelithiasis‚ choledocholithiasis‚ and early obstructed jaundice
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Enzyme Study Questions 1. Definitions/terminology: o enzyme: a protein molecule that catalyzes chemical reactions without itself being destroyed or altered o catalyst: a substance that increases the rate of a chemical reaction but is not consumed or changed by it. o substrate: a substance upon which the enzyme acts. o denaturation: the partial or total alteration of the structure of a protein without change in covalent structure by the action of certain
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One of the most terrifying and frightening words in the English language is cancer. Many children and adults of all ages can be diagnosed with a rare type of bone cancer each year. The harmful effects of this disease cannot only be detrimental but deadly too. Thus‚ with early detection‚ diagnosis‚ and proper treatment‚ individuals have a better chance of survival when treated early. First‚ bone cancer is a deadly disease that consists of malignant tumor that forms in the bone. It can also destroy
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Calcinosis cutis is rare condition signified by calcium deposition in skin and subcutaneous tissues. In all cases of calcinosis cutis‚ insoluble compounds of calcium are deposited within the skin due to local and/or systemic factors. These calcium salts consist primarily of hydroxyapatite crystals or amorphous calcium phosphate. It is commonly seen in blacks‚ and no apparent gender predilection is noted (6‚7) From the pathogenesis point of view‚ the condition is classified as metastatic‚ dystrophic
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Determination of the activation energy of an enzyme catalysed reaction Introduction In this practical the aim for this experiment was to find out the catalytic power of alkaline phosphate‚ as well as the rate of reaction and the activation energy of p-nitrophenol phosphate. Enzymes are biological molecules that catalyse a chemical reaction. ‘Enzymes work by lowering the activation energy of a chemical reaction making it easier to proceed’ [1]. This allows molecules to have more energy therefore
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Capparis spinosa L. belongs to the family Capparaceae is distributed throughout India and commonly known as Maratimokku in Tamil. In the present study attempts were made to assess the phytochemical‚ nutraceutical and chemical composition‚ pharmacological properties and safety profiles of C. spinosa. Powder microscopy revealed characteristics features of C. spinosa. Preliminary phytochemical screening of flower buds of C. spinosa L. revealed the presence of alkaloids‚ reducing sugars‚ carbohydrates
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bacterial challenge have been implicated with the incidence and prevalence of caries. The delicate balance of demineralization and remineralization to which hard tissues are subjected to dwells on the level of salivary calcium‚ phosphorus and alkaline phosphatase. At the early stage of demineralization‚ salivary calcium and phosphorus diffuse into the subsurface causing a reversal. However in children with high caries risk‚ repeated or increased
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for children suffering from HPP. Asfotase alfa is an important advance for many patients with HPP‚ their families‚ and the medical community because it can effectively replace in the skeleton the deficient enzyme called tissue non-specific alkaline phosphatase‚” said Michael Whyte‚ M.D.‚“Without treatment‚ many newborns and infants with HPP fail to develop a normal rib cage and die from not being able to breathe‚ and young children with HPP can suffer from muscle weakness. Treatment with asfotase
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