Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
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2-methylcyclohexanol using 4:1 mixture of phosphoric and sulfuric acid. Gas Chromatography was then used to measure the products. Two products were formed. The theoretical yield of the alkene mixture was 3.914 g. The actual yield of the mixture was 0.73 g. The percent yield for the reaction was 18.65%. Peak A had an area of 0.3 mm2 and a percentage of 4.6 %. Peak B had an area of 6.21 mm2 and a percentage of 95.39%. Introduction The acid used speeds up the dehydration of the alcohols. This is an elimination
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PAPER and COLUMN CHROMATOGRAPHY REPORT 1. 2. The unknown code is an amino acid‚ Glycine. To justify the reason is because the retention factor is the same exact number as the Glycine. The data of the unknown shows everything to be exact with the data of the Glycine 3. The mobile phase is the more polar during the capillary action of the experiment. As soon as the paper touches the mobile phase‚ the solvent rises to the amino acids. This is where you can find polarity of the amino acids.
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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If only they knew what was in the mystery mixture. After a long time of experimenting‚ the 8th grade scientist came to a decision on how they are going to figure what is in the mystery mixture. Once they started‚ they all looked at its physical properties. Then the scientists thought about the chemical reactions‚ and how that will help the claim the scientists had. More than half of the scientists thought it was baking soda and citric acid. When combining baking soda and citric acid together‚ the
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Alcohol advertisements should be banned from TV Alcohol Advertisements is the promotion of alcohol beverages by alcohol producers through ads on TV and also in a variety of media. It is one of the most famous forms of marketing and it is one of the major businesses in today’s economy. The question is why alcohol advertisements should be banned from TV? One might say that children or young audience influence from observing parents’ behaviour and they are the significant role models to follow in
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Separation techniques LIQUID CHROMATOGRAPHY ‘THE ART OF SEPARATION’ CHROMATOGRAPHY – AN INTRODUCTION Chromatography is a technique through which a mixture of chemical components are separated‚ identified and determined accurately. This technique while provides a way for analytical separations‚ also useful for preparative techniques by which pure compounds can be obtained. Detector Signal Blue Compound Sample Injection + Mobile Phase Retention Time Red Compound It is i defined d fi d as a
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Chromatography of Commercial Analgesics Using the thin layer chromatography the major components of a commercial analgesic tablet will be indentified and purified. The active ingredients of the analgesic used were characterized by comparison with chromatograms of aspirin‚ acetaminophen‚ ibuprofen‚ mefanemic acid‚ and caffeine through the use of UV chromatography then the Rf value was calculated for each solvent. INTRODUCTION In this experiment‚ thin-layer chromatography (TLC) will be used
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Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly
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Separation of a Mixture Introduction: Mixtures are not unique to chemistry; you use and consume them on a daily basis. The beverages you drink each morning‚ the fuel you use in your automobile‚ and the ground you walk on are mixtures. Very few materials that you encounter are pure. Any material made up of two or more substances that are not chemically combined is a mixture. The isolation of pure components of a mixture requires the separation of one component from another. Techniques
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