Abstract Western blotting is an analytic technique used around the world for many reasons; detecting infections‚ diseases‚ and particular proteins in a tissue. When western blotting‚ first the proteins are extracted and undergo Gel Electrophoresis‚ next it goes through elecroblotting‚ and finally detects the proteins. When the results after every step is completed‚ the membrane is analyzed based on where the bands are present in the gel. It has been told that actin and myosin is present in fish
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WESTERN BLOTTING (Written Report) I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a
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The Western Ghats or the Sahyādri constitute a mountain range along the western side of India. It is a UNESCO World Heritage Site and is one of the eight hottest hotspots of biological diversity in the world. It is sometimes called the Great Escarpment of India. The range runs north to south along the western edge of the Deccan Plateau‚ and separates the plateau from a narrow coastal plain along the Arabian Sea. The range starts near the border of Gujarat and Maharashtra‚ south of the Tapti river
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“Western Wind” “Western Wind” by an anonymous Middle English lyric relates to A Farewell to Arms by Ernest Hemingway. In A Farewell to Arms Fredrick Henry has a stream-of-consciousness. In a stream-of-consciousness the writer approximates the rapid‚ often chaotic thought patterns of the character. Thinking about Catherine‚ in the rain‚ Henry refers to the poem "Western Wind”. The poet of “Western Wind” wishes that his heart would heal from the pain of not being with the person that he loves
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The Western Market‚ without south block‚ is an oldest market building in Hong Kong. Built in September 1844 ‚ it was located at 323 Des Voeux Road‚ Sheung Wan. Let’s introduce some historical background about the western market After building the North block‚ the South Block of the Western Market was built in 1858. Sheung Wan residents buy fresh meat like pork‚ fish and chicken in the North Block. Then‚ the fruit and vegetable were sold in South Block. It was initially a very shabby market
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Weather Fronts: Understanding the Movement of Air Lesson Plan on types of weather fronts with various conceptual demonstrations By: Craig O’Connell and Jack Hord A. State Standards Addressed a. Standard 6.1: The student will demonstrate an understanding of technological design and scientific inquiry‚ including process skills‚ mathematical thinking‚ controlled investigative design and analysis‚ and problem solving. i. Indicator 6-1.1: Use appropriate tools and instruments
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Physical education (often abbreviated Phys. Ed. or P.E.) or gymnastics(gym or gym class) is a course taken during primary and secondary educationthat encourages psychomotor learning in a play or movement exploration setting. Physical education trends have developed recently to incorporate a greater variety of activities. Introducing students to activities like bowling‚ walking/hiking‚ or Frisbee at an early age can help students develop good activity habits that will carry over into adulthood.
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Context response: Write Fowler’s news article reporting on events at Phat Diem. Vietnamese Massacre Death is something we all fear and scared by. I was reminded of this on my recent travels to Phat Diem‚ “[I] didn’t want to be reminded of how little we counted‚ how quickly‚ simply and anonymously death came”. I bore witness to the death of many innocent souls; the death of civilians caught in the crossfire. My thoughts ran wild with questions‚ as everywhere I looked there was bloodshed and
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Morning/Afternoon/Evening etc. Informal: what is up‚ long time no tee‚ yeah. Guiding to Front Desk/Reception Counter: Bellboy comes into the scenario. His prime function‚ after greeting is to escort the quest to front desk. After that to guest room it is also called “Room the Guest” Registration: Registration procedures be gins at Front Desk/Reception counter. Front desk personal have to have ready smile at all the times while greeting the guest. As the guest approves the reception he/she must
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Physical Therapy Physical therapists held about 198‚600 jobs in 2010. Physical therapists‚ sometimes referred to as PTs‚ help people who have injuries or illnesses improve their movement and manage their pain. Physical therapists work as part of a healthcare team‚ overseeing the work of physical therapist assistants and aides and consulting with physicians and surgeons and other specialists. Physical therapists also work at preventing loss of mobility by developing fitness- and wellness-oriented
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