Pt requires 20 gauge IV for _______. 20 gauge catheter inserted to the right anterior forearm‚ first attempt. Flushed with 10 cc normal saline. Stat lock in place‚ covered with transparent dressing. Pt tolerated well with no complaints of pain or irritation upon flushing‚ no visible swelling or bruising. Sharps placed in approved container‚ patient’s bed lowered as far as possible and assisted to comfortable position. Reassessed in 5 minutes for bleeding ‚ none noted. Pt requires IV discontinuation
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Black Jews of South Africa: biological and cultural constructions of identity November 19‚ 2002 Text: 11 pages Figures: 2 pages Bibliography: 3 pages Introduction Walking through the Venda Plaza shopping center in Thohoyandou‚ South Africa‚ R dai ae ta a ad a tm ‚T aioe f Jws bo e . Gv g i a uznw vdo m n n si o e“ hts n o my e i rt r” i n h d h hs i m pzl ytn i e l kh ep i d“a a l k e . cm f m I al l gi e uz d ei r ud o ‚e xln ‚Im Ba Jw We a er s eao t e tg o ae c o r n m aoT e hv poe iwt
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Presence of Alu element within PV92 on chromosome 18 in a mixed ethnical population and its connection with humans’ ancestral origins By Shiyu Song Biology 155Q LAB-A: 14:30-17:30 Dr. Jacob April 17th 2013 Abstract The Alu insertion (+) within PV92 on chromosome 16 usually reflects a person’s ancestral origin. This study aimed to find the correlation between the presences of Alu insertion (+) within PV92 on chromosome 16 within a population. We used PCR to detect the Alu insertions by amplifying
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microevolution of Alu element TPA-25 was tested in the experiment through the examination‚ observation‚ and analysis of population alleles distribution under the Hardy-Weinberg Theory of Genetic Equilibrium. Alu elements affect the genome by causing insertion mutations‚ recombination between elements‚ gene conversion‚ and alterations in gene expression. In the lab PCR was used to amplify a short piece of DNA from human genome which allowed us to look for a DNA sequence called an Alu element. Electrophoresis
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The Polymerase Chain Reaction and Determination of Alu Population Freqencies Porshia Gibbs April 8‚ 2010 Genetics Laboratory Abstract Cheek cells were extrapolated and used in PCR amplification and electrophoresis of the amplified samples to determine the presence or absence of the dimorphic Alu sequence in a class population. A bioinformatic allele server was then employed to calculate genotypic and allelic frequencies of the Alu element in the class population. The Hardy-Weinberg equation
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Chapter 12 Genomes Study Guide By: Divya Prakriya Concept 12.1 : There are powerful methods for sequencing genomes and analyzing gene products. • The goal of sequencing genomes is to identify mutations in DNA and relate them to phenotypes (ie. Understanding genetics) • Human Genome Project- 13 year project‚ used chemically modified nucleotides • Next generation DNA sequencing- uses miniaturization techniques 1st developed for electronics industry‚ as well as principles of
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experiment‚ I intended to determine whether the sample population consisting of my fellow biology lab classmates would fall in the Hardy-Weinberg equilibrium with respect to the ALU insert from human chromosome 8. My hypothesis was that this sample population would fall in the Hardy-Weinberg equilibrium with respect to the ALU insert. To analyze whether this sample population was in the Hardy-Weinberg equilibrium or not‚ I amplified a sample DNA polymerase from a cheek cell
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The four forces of evolution include mutation‚ gene flow‚ genetic drift‚ and natural selection. Mutation is when there is a change occurs in a gene or chromosome structure which results in a difference in a physical or behavior trait. This trait can be carried into the next generation because the trait is contained in the gene. Gene flow is the process of genes transferring from one population to another. Genes can be transferred to different populations by migration of individuals along with the
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of the gel after electrophoresis. Gel 1 Gel 2 Lane 7. This is Maddie’s (MCB) sample. Gel 2 Lane 6. This is Madi’s (MN) sample. From our sample of the gel electrophoresis‚ both Madi and me are homozygous positive (+/+) for the Alu gene. This can be determined by looking at the ladder and comparing our sample to it‚ to find out if we are homozygous or heterozygous. Discussion For this lab‚ DNA from our cheek cells were separated through PCR‚ and singled out through gel electrophoresis
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Restriction Fragment Length Polymorphism (RFLP) Restriction Fragment Length Polymorphism (RFLP) Definition Definition In molecular biology‚ restriction fragment length polymorphism‚ or RFLP is a technique that exploits variations in homologous DNA sequences. It refers to a difference between samples of homologous DNA molecules that come from differing locations of restriction enzyme sites‚ and to a related laboratory technique by which these segments can be illustrated. In RFLP analysis
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