albumin were calculated with the interpretation of a standard curve. To determine the concentration of total protein in human serum‚ a total protein assay was performed by using the Biuret reagent. Proteins react to this compound because it contains two amide bonds. When the Biuret reagents is introduced to a sample of protein‚ it forms a blue or violet complex. The concentration of albumin in human serum is determined by using the Bromocresol green (BCG) reagent. When albumin and the BCG reagent are combined
Premium Protein Chemistry Serum albumin
Electrophilic Aromatic Substitution Objective The objective of this experiment was to illustrate electrophilic aromatic substitution by synthesizing p-nitroanilide (as well as ortho) from acetanilide by nitration. The para form was separated from the ortho form based on solubility properties using recrystallization techniques. Synthetic equations: Physical Properties & Hazards of Reagents/Products: (all taken from Sigma-Aldrich website) Acetanilide MM = 135.16 g/mol Melting point =
Premium Electrophilic aromatic substitution Benzene Sulfuric acid
ortho product was made to a larger portion‚ note o-nitroacetanilide melts at 90-94oC.5 Scenarios where the o-nitroacetanilide is synthesized with high yields is when acetyl nitrate attacks by a proposed reagent-substituent coordination between the amide and nitrate.3 Acetyl nitrate is typically generated from HNO3 and acetic anhydride‚ however it is suspected acetic acid with HNO3 caused its formation.3 Large excess of acetic acid was used to dissolve the acetanilide as it was challenging to dissolve
Premium Chemistry Chemical reaction Oxygen
acetaminophen as a potential substitute for a patient who is allergic to aspirin. Acetaminophen‚ a common analgesic drug that can be bought over the counter‚ was synthesised by Hormon Northrop Morse. With acetaminophen consisting of a benzene ring‚ an amide and an alcohol group‚ it has the ability to stop pain and reduce fevers. However‚ due to the state of technology‚ it is not clear as to how and what acetaminophen does to the body in order to have its antipyretic and analgesic effects. Many proposed
Premium Aspirin Paracetamol Ibuprofen
Analysis of Alcohols‚ Aldehydes and Ketones Karl Wayne Mancao‚ Raphaell Mordeno‚ Andres Pastrana III*‚ and Shannen Peñaverde Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The proponents have done several tests for identifying alcohols‚ aldehydes and ketones. These tests are Dichromate test‚ Tollens test‚ Lucas test‚ DNPH test and Iodoform test. Three samples got positive result in dichromate test and one in Tollens test. Lucas test got one sample that has
Premium Alcohol Oxygen Aldehyde
LABORATORY REPORT PRACTICAL 2 (ELECTROPHORESIS OF SERUM PROTEIN) DATE : 10 OCTOBER 2013 PART A SEPARATION OF SERUM PROTEINS USING THE ELECTROPHORESIS METHOD. OBJECTIVES: 1. To understand the analytical methods involved in analyzing serum proteins. 2. To study the serum protein electrophoresis pattern to aid the understanding of the relationship between the structure and the function of proteins. PRINCIPLE OF PRACTICAL: This technique is based on the movement of
Premium Pentose phosphate pathway Hematology Oxygen
1) 1) Disregarding stereoisomers‚ how many different enols can the β-diketone CH3 COCH 2 COCH 2 CH3 form? A) 3 B) 1 C) 4 D) 0 E) 2 2) Methylamine reacts with acetophenone to yield the: A) imine. B) enamine. C) acetal. D) amide E) iminium salt. 2) 3) What product results when an aldol is dehydrated? A) β-diketone B) β-ketoester C) conjugated alkyne D) β‚γ-unsaturated aldehyde E) α‚β-unsaturated aldehyde 3) 4) Which of the following will react most slowly with an enamine
Premium Prime number Harshad number
Methoxyflurane a. IV i. Dizzepam ii. Loraepam iii. Midazolam (drug of choice for IV) Local anesthetics (esters and amides) End in –caine (lidocaine‚ buplvacaine‚ procaine‚ cocaine) Be familiar with the potential adverse effects of general and local anesthetics Local: hematologic toxicity-cyanosis chocolate colored blood‚ Allergic reactions- acid derivatives‚ rare with amides Tinnitus‚ agitation‚ restlessness‚ seizure activity‚ decreased cardio ecitation‚ fatigue dizziness General adverse
Premium Benzodiazepine Morphine Anesthesia
enzymatic hydrolysis [14]. 2-AG is hydrolyzed and deactivated primarily by monoacylglycerol lipase (MAGL) also through a recently described serine hydrolase a/b-hydrolase domains‚ ABHD6 [20-21]. Anandamide hydrolyzed and degraded mainly through fatty acid amide hydrolase (FAAH) [19‚ 22]. It has been shown that FAAH and MAGL inhibitors intensify endogenous eCB activity with temporal and spatial fidelity and should be superior to direct eCB agonists as therapeutic agents
Premium Drug addiction Opioid Pharmacology
a denaturation process. Denaturation disrupts the normal alpha-helix and beta sheets in a protein and uncoils it into a random shape. Denaturation occurs because the bonding interactions responsible for the secondary structure (hydrogen bonds to amides) and tertiary structure are disrupted. In tertiary structure there are four types of bonding interactions between "side chains" including: hydrogen bonding‚ salt bridges‚ disulfide bonds‚ and non-polar hydrophobic interactions. which may be disrupted
Premium Protein Amino acid