The Effect Of Enzyme Concentration On Enzyme Activity The pancreatic duct in individuals who have cystic fibrosis frequently becomes blocked‚ reducing or preventing the release of pancreatic enzymes into the small intestine. The aim of this activity is to investigate the effect of a reduction in enzyme concentration on the rate of reaction‚ in this case the breakdown of protein by protease enzymes. Aim – Milk powder contains a white protein called casein. A white suspension of
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concept and meaning of pH 2. Provide the student experience in measuring pH a. pH testing paper 3. Test the student’s hypothesis as it related to the pH of common solutions Hypothesis The pH of the tested solutions will be in the order of the following according to a pH scale: 1. Lime juice 2. Orange juice 3. Soda 4. Iced Tea 5. Milk 6. Water 7. Soapy water Material Required To facilitate this laboratory exercise‚ the experimenter needs the following: pH strips Sample reservoirs
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Properties of pH The manufacture’s claim is that Sensodyne toothpaste micro hardens tooth enamel to help protect against the effects of acid wear‚ helps stop the twinges of pain from sensitive teeth formulated with low abrasivity‚ neutral pH and gentle yet effective cleaning system. To find out if the manufactures claim on Sensodyne toothpaste is the correct level of pH. I expect the Sensodyne toothpaste to be a neutral pH level as the manufactures claim that this products pH level is neutral
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enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To test the optimal pH‚ the starch and a buffer were combined at a specific pH level and
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are amylases? Amylases are enzymes that break down starch or glycogen. Amylases are produced by a variety of living organisms‚ ranging from bacteria to plants and humans. Bacteria and fungi secrete amylases to the outside of their cells to carry out extracellular digestion. When they have broken down the insoluble starch‚ the soluble end products such as (glucose or maltose) are absorbed into their cells. Amylases are classified based on how they break down starch molecules i. α-amylase (alpha-amylase)
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What amount of amylase is the best to produce a better reaction with starch? A 1% solution of amylase‚ made from 0.15g of amylase added to 15ml of water‚ a 2% solution of amylase made of 0.3g of amylase added to 15ml of water‚ and a 0.5% solution of amylase made of 0.75g of amylase to 15ml of water were created. A 0.25% starch solution was also created‚ and 3ml was added to each of the 4 test tubes. 1 drop of iodine was added to each test tube. 4ml of the 1% solution was added to the first test
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Experiment 3: Soil pH measurement Introduction Aims The aims of the experiment were to determine the pH of a variety of soils which included sedentary‚ 3:2‚ sand‚ organic matter with the means of a pH meter at various soil water ratios and with or without calcium chloride. Method and Materials Samples of sedentary soil‚ 3:2 soil‚ sand‚ organic matter and compost‚ 100ml vials(x12)‚ bottle of distilled water‚ analytical balance‚ pH meter‚ 2 buffer solution of known pH‚ 0.25M calcium chloride
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Determination of pH Almira‚ Faerie Carleen Lucile L. Gallardo‚ Charlotte O. Group #6‚ Chemistry 18.1‚ MHEG1‚ Ma’am Arlou Angeles September 23‚ 2013 I. Abstract The acidity of the four unknown solutions were determined with the use of colorimetry using McIlvaine’s buffer solutions varying in proportion of its constituents (disodium phosphate and citric acid). These buffer solutions were subjected to the addition of corresponding pH indicators and the variation of colors depending on its pH level was
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can reach a new pH level by interacting with soil. If water has a pH that is too high or too low‚ it can be life threatening to aquatic species and can corrode metal structures. After finding this out‚ I decided to test if water could reach an extreme pH just by interacting with soil around it. The question for this experiment therefore became the following. Will interaction with soil change the pH of water? I hypothesized that if water interacts with soil for three days‚ then the pH of the water will
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Introduction: The purpose of this lab was to check the time needed for salivary amylase and phosphorylase to produce a negative result and how different concentrations affected those times. Enzymes are biological catalysts that can cause a specific chemical change in any part of the body (Walsh‚ 2002). Many of the reactions that take place within a cell would normally take place at temperatures substantially higher than those present inside a cell (Alberts et al.‚ 2010). Because of this‚ these chemical
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