Experiments investigating the effect of a particular factor on an enzyme-catalysed reaction are commonly used as assessed practicals in Biology. The reason for this is there are a number of factors that can be investigated‚ and therefore‚ a number of factors that can also be controlled. From our lecture‚ we are aware that the following can affect the rate of reaction: 1) Temperature: a. Independent variable: A range of temperatures should be investigated‚ with particular attention
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Biology Lab Report Research Question: Effects of pH on amylase activity Introduction: Amylase is an enzyme that is in human’s saliva as well as the pancreas. Enzymes are biological catalysts that speed up a chemical reaction. They break down complex molecules into simple ones. In this case‚ amylase converts starches (complex molecule) into simple sugars. That is why foods like potatoes for example‚ may taste sweet to us‚ because they contain starch. The optimum pH for pancreatic
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For this experiment‚ we started off by taking tubes numbered 1-4 and started adding one scoop of our enzyme catalyst‚ in this case‚ the yeast. We then proceeded to measure and add 1 mL of distilled water to test tubes A-D. To get a more accurate measure of 1 mL of distilled water‚ we used the dropper labeled “W” to drop distilled water into the 5 mL graduated cylinder until we saw that the bottom of the water line reached closely to 1 mL. Next‚ we took the four tubes with the scoop of yeast and
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PLANNING COURSEWORK- STARCH AND AMYLASE AIM The aim of this coursework is to investigate the effect of temperature change‚ on the rate of hydrolysis of starch catalysed by amylase. PREDICTION I think that as the temperature increases‚ the rate of reaction also increases‚ to a point when it dramatically decreases. On graph 1‚ you will see a sketch of the graph which I expect to be the result of the experiment. SCIENCE REASONING I think my prediction is correct because the rate of
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CHAPTER 4: ENZYMES Enzymes are biological catalysts. There are about 40‚000 different enzymes in human cells‚ each controlling a different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures. They were discovered in fermenting yeast in 1900 by Buchner‚ and the name enzyme means "in yeast". As well as catalysing all the metabolic reactions of cells (such as respiration
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Pre-lab questions: 1. Algae belong to what Kingdom? They belong to the Kingdom Protista. 2. What are the three types of multicellular algae? The three types if multicellular algae are chlorophyta which are green algae‚ phaeophyta which are brown algae‚ and rhodophyta which are red algae. 3. Are algae autotrophs or heterotrophs? Algae are autotrophs. 4. What organelle allows plants (and plantlike protists) to photosynthesize? The chloroplasts allow plants and plantlike protists to photosynthesize
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Labs 4 & 5 Lab Four: Experiment 1 1. At what magnification do you first notice the ragweed pollen? I first noticed it when the magnification is at about 30 2. Which is bigger‚ rhinovirus or E. Coli? E. Coli is bigger 3. Based on the magnification‚ how many of the E. Coli can fit into the same space as the head of a pin? 10‚000 E. Coli 4. About how many red blood cells could fit across the diameter of a human hair. (Again‚ look at the magnification scale)? About 100 blood cells
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structure of the membrane. Phospholipids form a bilayer‚ which is the basic structure of the membrane. Their non-polar tails form a barrier to most water soluble substances. Membrane proteins serves as channels for transport of metabolites‚ some act as enzymes or carriers‚ while some are receptors. Lastly carbohydrate molecules of the membrane are relatively short-chain polysaccharides‚ which has multiple functions‚ for example‚ cell-cell recognition and acting as receptor sites for chemical signals.
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Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free
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pH for starch on amylase. The experiment was carried out in one day. The researcher and a partner did the experiment based on a lab manuel from class. Data was collected from the experiment and to be displayed on graphs. Then the optimum pH and temperatures were to be calculated based on the findings. The hypothesis was disproved due to the optimum pH of 5 but the other findings supported the hypothesis of the optimal temperature being 45 degrees Celsius. Materials In biology lab we conducted an
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