pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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DISCUSSION: Bromelian added to Gelatin: Bromelian is an enzyme found in pineapples. When Bromelian is added to gelatin it breaks down the protein and does not allow the gelatin to solidify. There are several factors that can cause an enzyme to slow down or to completely stop reacting. For example‚ temperature and pH can effect enzyme activity. Canned pineapple juice and fresh pineapple juice were used to see how the enzyme would react differently. In fresh pineapple juice the Bromelian have would
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of the lab is to see the effects of enzyme concentration on the reaction rate. Part 2: Effect of Substrate Concentration Purpose: The purpose of this part of the lab was to find the effect of substrate concentration on enzyme activity. Part 3: Effect of Temprature Purpose: The purpose of this part of the lab was to determine the effect of temperature on the rate of enzyme activity
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Lab Report: Enzyme Lab Your Name: Samantha Butcher Purpose of this Lab What is the goal of this lab? What question is it trying to answer‚ or what problem is it trying to explain? It is going to explain how a liver’s enzymes break down substances that could be harmful.| Hypothesis After reading the lab instructions - but before starting the lab - record your best “educated guess” about what will happen in the experiment. Give your reasons and outline any assumptions that lead
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Enzyme Activity Lab Hypothesis: 1] Are enzymes reusable? Yes‚ enzymes are reusable. 2] Which of the following do you think contain catalase? [Manganese dioxide‚ chicken meat‚ apple‚ carrot‚ potato‚ or chicken liver] I think that the manganese dioxide‚ apple‚ and carrot contain the catalase. 3] Would grinding the materials affect enzyme activity? I think that grinding the materials will increase enzyme activity. 4] Would temperature affect enzyme activity? I think that
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Sample Lab Report: Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include: background information about concepts involved in the lab‚ statement of purpose of the lab identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in
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The enzyme tyrosinase was successively extracted by combining a homogenate of a potato and sodium sulfate with ammonium sulfate. Tyrosinase was successfully extracted by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount
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Enzymes Abstract: The following 2 labs experimented the more enzymes and substrates added to the concentration will effect the reaction rate. Our second lab‚ we tested enzyme and substrate concentrations to determine the increase of temperature and inhibitor. The enzyme source used in both labs was peroxide‚ guaiacol is used as a substrate for peroxide. We used Guaiacol‚ turnip extract‚ peroxide and distilled water for enzyme and substrate concentration. In the second lab we used the same substances
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Enzyme Lab Marcus James. December 20‚ 2013 HL Biology 3 Period 1 Purpose and Background The purpose of this lab is to explain how enzymes act as catalysts for biological reactions in different temperatures. This lab relates to enzymes‚ proteins‚ and substrates; that we learned in class. The union of the enzyme and the substrate is called the enzyme-substrate complex. The make-up of an enzyme is proteins and made up of chains and amino acids. Enzymes
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Effect of the Changes in the Environment to the Functionality of the Enzymes Introduction a. Background In our everyday lives‚ enzymes are used in our bodies‚ and in nature around us‚ to speed up the chemical reactions happening constantly‚ which happens by lowering the amount of activation energy needed to start various reactions. The way this works is by attaching the particular substrate to the active site of the enzyme‚ where it will start to aid the chemical reaction. Then‚ the allosteric
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