Cavanaugh Lab: Tuesdays at 1pm Enzyme Kinetics Lab Introduction: Enzymes are proteins that will catalyze reactions to make the rate of the reaction occur faster than it would without. It can also make the reaction occur in the first place. Tyrosinase is an enzyme that has a variety of functions and activities. It produces pigments like melanin and others that would be apparent when a fruit is cut in half and it browns. (Bien-etre 3).There is that one function that stands out and the enzyme is continuously
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Investigation on the effects of Temperature‚ pH levels‚ and Enzyme Concentration on the reaction rate of the Decomposition of Hydrogen Peroxide by Catalase The purpose of this investigation is to figure out how temperature‚ pH‚ and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to
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Ah Seung Chong Molecular Biology CTW: Enzyme Kinetic Dr. Cruz 07/22/2010 Enzyme kinetics Introduction Enzymes are biological catalysts or assistants‚ without enzyme many of important processes of life could not happen. Most of enzymes are proteins that help speed up chemical reactions by lowering amount of activation energy needed for the reaction1. Enzymes are usually highly selective‚ only bind to specific substrate and convert it to product at a particular rate1. The rate of the reaction
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The Effect of Temperature on Animal and Fungal Amylase’s Ability to Breakdown Starch. Abstract This experiment was designed to test the reaction of the enzyme amylase at various temperatures. There were two different kinds of amylase being tested‚ one was fungal amylase also known as aspergillus oryzae and human amylase. The changes in temperature effect the rate at which an enzyme and a substrate collide. When the temperature is too high the active site changes shape or denatures‚ once this
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1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzyme‚ concentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚
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Introduction Enzymes are proteins that are involved in all the chemical processes in living things. As they are made of proteins they are affected by pH and temperature. Enzymes are catalysts; they speed up chemical reactions without being changed themselves. Digestive enzymes speed up the breakdown of large food molecules into smaller ones so that the blood can absorb them. Enzymes turn a large starch molecule into thousands of tiny glucose molecules. Enzymes end in ’ase’. There are thousands of
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ENZYMES LABORATORY REPORT Introduction The utilization of any complex molecule for energy by an organism is dependent on a process called hydrolysis. Hydrolysis breaks complex molecules into simpler molecules using water. Similarly‚ the process that is the reverse of this is called dehydration synthesis‚ which removes water from simpler molecules. However‚ because hydrolysis occurs very slowly‚ living organisms use biochemical’s called enzymes to speed up the reaction. In this lab exercise
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PH level of Amylase Background info: What is Amylase? Amylase is an enzyme that helps digest carbohydrates. It is produced in the pancreas and the salivary glands. (Dugdale & Longstreth‚ 2011) Factors Affecting Amylase: Things that affect the efficiency of Amylase are temperature and pH levels. (Wikimedia Foundation‚ Inc‚ 2013) Function in the body: The function of Amylase in the human body is to break down plant-based starch sources. Therefore‚ providing the human body with more
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Enzymes are catalysts that speed up chemical reaction but are not themselves consumed or changed by the reaction. The cell’s biological catalysts are proteins. Proteins are made up of one or more polypeptide chains that are folded to make an active site‚ an area in which a material to be acted on by the enzyme‚ called the substrate‚ will fit. The temperature‚pH‚ the concentration of enzyme‚ and the concentration of substrate all affect the activity of the enzyme and the rate of the reaction. The
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Varibles that affect Enzyme Catalysis Reaction Rates Introduction Molecules are constantly moving in our bodies and in nature. When molecules move fast enough they collide into one another‚ allowing chemical reactions to occur. Factors such as temperature and concentrations can either help increase or decrease these reactions. (Jubenville.) Enzymes are known as catalyst because they are able to speed up reaction rates without being destroyed or altered. They are able to encourage chemical reactions
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