Experiment 2: IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different
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This study focuses on the effect that temperature has on the enzyme amylase. Enzymes are composed of proteins and acts as a catalyst to speed up the rate of a specific chemical reaction by lowering the activation energy. Reactions do not need enzymes to occur‚ but the human body and other living organisms depend on the use of enzymes in order for biochemical reactions to happen in milliseconds. Each enzyme contains a distinct three dimensional structure which is related to its particular function
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Experiments in physics lab report Department of Electrical Engineering Isabela State University Ilagan Campus‚ Calamagui 2nd‚ City of Ilagan‚ Isabela baccaljoemel@yahoo.com Submitted to: Engr. Rafael S. Ramos Physics12 Professor Submitted by: Joemel L. Baccal BSEE IIA Experiments in Physics Lab Report Joemel L. Baccal Department of Electrical Engineering Isabela State University - Ilagan Campus‚ Calamagui 2nd‚ City of Ilagan‚ Isabela baccaljoemel@yahoo.com Abstract In
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FIRE RESISTANT BALLOON: Materials - 1 matchbox - 1 candle - 1 yellow colored balloon - 1 blue colored balloon - Half a cup of water Procedure: 1. For this experiment‚ the independent variable is whether the balloon is filled with water or not. The dependent variable is what happens to the balloon when it is placed above the lit candle. This is determined by observing the balloon. The constants (control variables) are how much the balloon is inflated and the amount
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process of distillation. Distillation is the separation of volatile substances mixed with nonvolatile substances by boiling the mixture to alter the phase of the volatile substance. The researchers used the simple distillation set up to conduct the experiment. 25 ml of The Bar Vodka was used and 7 ml of distillate was collected before a temperature of 95 degrees Celsius was reached. Collected distillate was then subjected to a flammability test to confirm ethanol content. The percent ethanol was computed
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Determining the Optimal Temperature and PH of Barley Amylase Abstract The purpose of this experiment was to find the optimal temperature and pH of barley alpha-amylase. I hypothesize that the optimal temperature would be 55 degrees Celsius and the optimal pH would be 5.5. In this experiment‚ the starch is used as a substrate to examine the optimum temperature and pH for the reaction of alpha amylase. It is known that the measuring of disappearance (absorbance) of the substrate starch with iodine
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Austin Vandermey GS-1140 April 4th‚ 2015 Experiment We as a society have one big problem on our hands. This problem is sleep deprivation because there are not enough hours in the day to get stuff done. Between work‚ school‚ family‚ and leisure it seems like we do not have enough time to get are daily tasks done let alone have time for sleep. It is recommended by the U.S Department of Health that people need a solid eight hours of sleep to maintain good health. I don’t know about you
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Industries‚ 2011. The experiments included within this lab manual are suitable for supervised or unsupervised learning environments. eScience Labs assumes full liability for the safety and techniques employed within this manual provided that all users adhere to the safety guidelines outlined in the mandatory eScience Labs Safety Video‚ Preface‚ and Appendix. All users must understand and agree to the eScience Labs safety guidelines prior to beginning their lab experiments. eScience Labs does not
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EXPERIMENT 5 REDOX TITRATION: TITRATION USING SODIUM THIOSULPHATE Objectives 1. 2. To prepare a standard solution of potassium iodate for use to determine the concentration of sodium thiosulphate solution accurately. To acquire the proper techniques of carrying out a titration. Introduction Redox titrations using sodium thiosulphate as a reducing agent is known as iodometric titration since it is used specifically to titrate iodine. The reaction involved is: I2 + 2Na2S2O3 I2 + 2S2O322NaI + Na2S4O6
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Experiment : 1 Tittle : Preparation of bis(acetylacetonato)copper(II) complex Objective : To synthesis the bis(acetylacetonato)copper(II) complex Introduction : A complex ion is usually form with high charge density metal ion as a central and formation of coordinate covalent bond (dative bond) with high electron molecules or ions. These molecules or ions are functioning as ligands (electrophile in organic compounds). They are easily attracted by electrophile (electron deficiency
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