THE EFFECT OF TEMPERATURE ON CATALASE RESEARCH QUESTION: What is the effect of various temperatures‚ 0°C‚ room temperature‚ 37°C‚ 50°C‚ 60°C‚ on the number of oxygen gas bubbles liberated‚ in a decomposition reaction between the enzyme Catalase‚ obtained from crushed mung beans‚ and 2% of the substrate Hydrogen Peroxide? INTRODUCTION: Enzymes are biological catalysts that increase the rate of chemical reactions without they themselves being involved in the reaction itself. Enzymes are proteins
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Introduction For this experiment‚ we are going to determine the effect of temperature on solubility‚ to be done in a chemical by dissolving a solute in a definite amount of solution which is saturated. Specifically‚ the goal of this experiment is to prepare a saturated solution of Na2C2O4 in water at different temperatures‚ determine the effect of temperature in solubility‚ and to apply Le Chatelier’s Principle. We can do all this by simply titrating a certain amount of standard KMnO4‚ and measuring
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Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through? 50‚ 100‚ and 200 4. Which materials diffused from the left beaker to the right beaker? NaCl‚ Urea‚ and Glucose at MWCO 200 5. Which did not? Why? Albumin‚ too large to diffuse Activity 2: Simulating Dialysis 1. What happens to the urea concentration in the left beaker
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Membrane Behavior Lab Abstract: Introduction: The permeability of a cell to solutes in an aqueous medium depends upon the physical and chemical make–up of the membrane. The maintenance of the living cell depends upon the continued presence and functioning of a selectively permeable membrane. If the nature of the membrane is changed or altered in any way‚ this may well affect its permeability and thus the properties of the cell of which it is a part. Irreversible changes in the permeability
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LAB 9: DATE: 25TH January‚ 2011. FORM CLASS: L6 3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of temperature on the enzyme lipase INTRODUCTION: The phenomenon of catalysis makes possible biochemical reactions necessary for all life processes. Catalysis is defined as the acceleration of a chemical reaction by some substance which itself undergoes no permanent chemical change. The catalysts of biochemical reactions are enzymes and are responsible for bringing about almost
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The Effect of Temperature on Beet Cell Membranes Introduction In this lab‚ we are going to learn how the stress of temperature affects fresh beets. We have come to learn that cell membranes organize the chemical activities of cells. All cells are made of plasma membranes‚ often called fluid mosaics. It is sometimes described as a mosaic because it is made of protein molecules that are embedded into phospholipids. Phospholipids are the main structural support of the membrane and the proteins
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Biological Membranes Lipid Membranes • Receptors‚ detecting the signals from outside: Light Odorant Taste Chemicals A Hormones Neurotransmitters Drugs • Channels‚ gates and pumps • Electric/chemical potential Neurophysiology Energy • Energy transduction: Photosynthesis Oxidative phosphorylation • • • • • • Structure Function Composition Physicochemical properties Self-assembly Molecular models highly selective permeability barrier Internal membranes for organelles Bilayer Permeability
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test for the effects of temperature on catalase activity. Introduction: Enzymes are proteins that act as catalysts and help complex reactions to occur everywhere in life. Enzymes affect a chemical reaction by acting as a catalyst‚ which is to say‚ it speeds up the reaction. However‚ extreme ph and temperature hinders enzyme function. Optimal activity of an enzyme is the optimal environment that an enzyme requires to function‚ which means having the optimum ph and temperature to catalyze a
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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ACTIVITY ONE- Cell Transport Mechanisms and Permeability: Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. Molecular weight affects the rate of diffusion because the bigger the size of the molecule the longer it will take to diffuse. Membrane size is another variable that affects the rate of diffusion because if the membrane’s pores are small it will take molecules longer to diffuse though it than if the pores were larger. Also‚ membrane thickness
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