Experiment 5 Title: Membrane Permeability Objectives: 1. Define solvent‚ solute‚ solution‚ selectively permeable‚ diffusion‚ osmosis‚ concentration gradient‚ equilibrium‚ turgid‚ plasmolyzed‚ plasmolysis‚ turgor pressure‚ tonicity‚ hypertonic‚ isotonic‚ hypotonic; 2. Describe the effects of hypertonic‚ isotonic‚ and hypotonic solutions on Elodea leaf cells and onion scale leafs. Introduction: Membrane permeability is a quality of a cell’s plasma membrane that allows substances to pass in
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substances across a semipermeable membrane to gain a better understanding of how cells move materials across cell membranes. The experiment was designed to exhibit Brownian Motion; the random movement and tendency of particles of a substance to move from an area of greater concentration to lower concentration via diffusion until equilibrium has been attained. In addition to observing the passive process of diffusion‚ the experiment demonstrated how a semipermeable membrane will only allow certain substances
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Planning AIM Beetroot cells contain a red pigment‚ which is stored in the cell vacuole and a vacuole membrane to prevent this leaking out of the cell surrounds it. The outer of the cell is also surrounded by a membrane‚ which again helps contain the pigment inside the cell. In this experiment I aim to find out the relationship between the leakage of red pigment from a beetroot cell and the surface area. To do this successfully I will need to alter the surface area of the beetroot cells accurately and then
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Aim: To investigate how effect of Detergent Concentration (cont.) has on Membrane permeability of Beetroot cells. Hypothesis: I predict that as detergent concentration increases‚ the solution will become less clear‚ plus mass increases. The increases in mass will indicate that the water potential of the Beetroot cell is lower than that of the surrounding sucrose solution. The Beetroot discs will become flaccid and decrease in mass if the water potential of the surrounding solution is lower than
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Purpose: Examine the role of the cell membrane in the cell by disrupting its function using temperature (Biology 107 Laboratory Manual 2014). This will improve the general understanding of optimal growing temperatures and the breakdown of the cell membrane Procedure: Betacyanin solution of a known concentration was diluted to create a dilution series‚ then placed in a spectrophotometer set to 525 nm. The absorbance of the dilution was used to create a standard curve for betacyanin. Discs of living
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Investigation the effect of the organic solvents on the cell membrane of the beetroot Objective: To investigate the effect of different organic solvents‚ such as alcohol and paraffin oil‚ on the cell membrane of the beetroot by using the red pigments in the beetroot as indicator. Hypothesis: Organic solvents dissolve the organic matter in the cell membrane (such as phospholipids). This destroys the cell membrane‚ and the permeability of the cell membrane is disrupted casing red pigments to
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Implementation My aim for this experiment was to find the effect of temperature on the membrane permeability of beetroot. Since I was unfamiliar with this experiment‚ I first conducted a preliminary test. I considered my preliminary test as my trial for this experiment. My Hypothesis for this experiment was to found out the amount of pigment loss of beetroot when exposed to varying temperatures. Betalain pigment found in beetroot My method for this experiment is:- * Use a cork borer to cut about
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EFFECT OF CHANGES IN TEMPERATURE AND SDS CONCENTRATION ON THE STRUCTURE OF BEETROOT CELL MEMBRANES. Introduction Cellular Membranes or cell membranes are biological membranes that separate the interior of a cell from the outside environment. Cell membranes are selectively permeable to ions and some molecules and control the movement of substances in and out of the cell. (Danyk‚ 2013/14) Cell membranes separate and organize chemicals and reactions within cells by allowing selective passage
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whether different temperatures will affect the cell membrane‚ thus would then releases the purple pigments out of the vacuole which causes the leakage of the purplish liquid. Background Information The outermost layer is the cell wall‚ which is present only in plant cells and is made up of a carbohydrate called cellulose and also has other protein substances embedded within it. The cell wall is a rigid layer and gives structural stability to the cell and also limits the permeability of large substances
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Usman Omid Biology IB 1A The Effect of Temperature on Beetroot Membranes _Aim_ This practical will test the effect of temperature on the integrity of the membranes‚ _Introduction_ The cells of beetroot contain a pigment called betalain in their vacuoles. It is kept inside the cells by the membranes. If these membranes are damaged‚ then the betalain leaks out. The amount that leaks out can be assessed‚ as the leaked out pigment will color the water surrounding the cells. This information can be used
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