filter flask with side tube‚ three glass capillary tubes‚ a set of melting point apparatus‚ one long hollow glass tubing‚ one electronic balance‚ one heater and the oven Background: Stereochemistry is always encountered throughout organic chemistry. And stereochemistry isomerism can be divided into two classes‚ geometrical isomerism and optical isomerism. Although these categories are not mutually exclusive‚ it is not uncommon to find compounds that exhibit only one or the other of the
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Analytical Science 2 Practical 2: High Pressure Liquid Chromatography Results Retention times should be quoted to two decimal places and standard deviations to three significant figures. Retention times (mins): Anthracene 8.67 Benzene 4.37 Naphthalene 5.98 Anthracene standards Peak Area Ratio Sample Concentration Anthracene Benzene A/B 4 2.25 mg/100mL 355003 496920 0.714406746 3 4.5 mg/100mL 583453 467385 1.248334885 2 6.75 mg/100mL 888620 513249 1.73136236
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A simple‚ specific‚ accurate and rapid reversed phase high performance liquid chromatographic method was developed for the simultaneous estimation of cilnidipine and chlorthalidone‚ using a Hiber® C18 (25cm x 4.6 mm)‚ Lichrospher® 100‚ RP-18 e (5µm) column and a mobile phase composed of phosphate buffer(pH 4.0 adjusted with orthophosphoric acid) : Acetonitrile (30:70‚ v/v). The retention times of Cilnidipine and Chlorthalidone were found to be 3.967 min and 6.427 min‚ respectively. Linearity was
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milk sample and to use dry method extraction specifically the soxhlet method. Methodology Chemicals and Apparatus The materials and apparatus that were used in the experiment were: Soxhlet‚ reflux condenser‚ extraction flask‚ analytical balance‚ iron stand‚ stove‚ filter paper‚ stove‚ spatula‚ milk sample‚ petroleum ether‚ and rubber hose. Procedure Two grams of milk sample was weighed and was placed into
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1. What is Chemistry? The study of the composition‚ structure‚ and properties of matter. The processes that matter undergoes and the energy changes that accompany these processes. 2. What is the formula for density? If I give you the density of a substance and the volume‚ you should be able to tell me the mass… Density = Mass/Volume 3. Compare and contrast intensive and extensive properties. Give an example of each. Extensive properties depend on the amount of matter that is present. Such
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References: Antharavally B.S‚ Bell P.A‚ Haney P‚ Mallia K.A‚ Rangaraj P. 2008. Quantitation of proteins using a dye–metal-based colorimetric protein assay. Analytical Biochemistry. 385; 342-245. Boyer R‚ 2000. Modern Experimental Biochemistry‚ third edition. Addison-Wesley Longman‚ Inc. USA. (41-45). Lambert J.B‚ Gronert S‚ Lightner D.A‚ Shurvell H.F‚ 2011. Organic Structural Spectroscopy‚ second edition. Pearson
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Romero 1 Kate RomerO Mr. Sousa Organic Chemistry ACL 8 January 2015 Chromatography Chromatography is a physical method of separating substances based on their properties‚ by distributing their components between a mobile and stationary phase. Chromatography is useful for observing mixtures and solvents‚ since it can be used to determine the relative bond strength of various compounds‚ a substances phase‚ and it can also the identity of unknown substances. Chromatography allows for the separation
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Name__________________________ Chemistry 190: Organic Chemistry EXAM 3 Thursday April 21‚ 2011 1. (12) ______ 2. (14) ______ 3. (20) ______ 4. (12) ______ 5. (54) ______ 6. (16) ______ 7. (12) ______ 8. (10) ______ Total (150) ______ The exam consists of twelve numbered pages and an unnumbered cover sheet. Make certain that you have a complete exam. You will have two hours to work on the exam. No books or notes are allowed; however‚ you may use a molecular model set and a calculator
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Figure 3.1: Typical standard chromatogram of Ritonavir and Lopinavir Figure 3.2: Typical sample chromatogram of Ritonavir and Lopinavir 3.3.2 Method validation The optimized RP-HPLC method for simultaneous assay of Ritonavir and Lopinavir was validated according to ICH guidelines with respect to system suitability‚ linearity‚ sensitivity‚ accuracy‚ precision and robustness. 3.3.3 System suitability Before sample analysis‚ the chromatographic parameters used in this analysis must conform the system
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References: Martin‚ S. (2012) BIOL 1F90 Lab Manual 2012 – 13 Ed. Brock University‚ St. Catharines‚ Ontario‚ Canada. Zor‚ T.; Selinger‚ Z. (1996) Linearization of Bradford Protein Assay Increases It’s Sensitivity: Theoretical and Experimental Studies. Analytical Biochemistry‚ 236: 302-308.v
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