BASIC SEPARATION TECHNIQUES Contemporary modifications of filtration and dialysis use a matrix-based fibrous material that provides a mechanism of separation in many homogeneous immunoassays. These materials may be coated with specific antibody-ligand to foster selection of specific materials or species. Certain labels use magnetic particles in conjunction with strong magnets to effect separation. Basic universally used separation mechanisms‚ outside of those incorporated in immunoassay‚
Premium Filter paper Analytical chemistry
Band broadening theory (Van Deemter equation) It is well recognized now that column band broadening originates from three main sources: 1. multiple path of an analyte through the column packing; 2. molecular diffusion; 3. effect of mass transfer between phases. In 1956 J.J. Van Deemter introduced the equation which combined all three sources and represented them as the dependence of the theoretical plate height (HETP) on the mobile phase linear velocity. Originally‚ it was introduced for gas
Premium Chromatography Gas chromatography Analytical chemistry
The Role Of Chemistry To Our Society Chemistry plays a huge and important role in society‚ with relation to shelter and clothing. For example‚ chemists come up with new paints and epoxy sprays that protect shelters from the wear and tear inflicted by rain‚ wind‚ sun and pollution. These space age coatings and paints are designed to make properties look better‚ perform better and last longer. Thermal chemical compounds may protect rooftops and also help structures stay at the right temperature
Free Chemistry
| | |CHAPTER 1: INTRODUCTION TO CHEMISTRY | | |2 |1.1 Understanding chemistry and |A student is able to:
Free Atom Periodic table Chemical element
AP Chemistry Kinetics Lab Abstract: The purpose of this lab is to learn how to write law expressions‚ determine orders by graphs‚ and calculate rate constants. By determining the number of drops of sodium hypochlorite necessary to make a diluted food dye change to colorless in less than three minutes helps calculate the absorbance which can then be analyzed to find the pseudo rate constant and eventually leading to the rate constant and the rate law. The results show that the m and n are both 1st
Premium Chemistry Chemical reaction Reaction rate
Chemistry 02 Oct 2014 Flame Test Lab Purpose: To find out the unknown compound from known compounds and the color of the flame. Safety & Precautions: •Do not cross contaminate compounds. •Keep goggles on at all times when working on lab. •Long hair should be tied up to avoid being singed. Table of Observations Compound Flame Color Strontium Chloride strong dark orange red Strontium Nitrate bright orange Copper
Premium Chemistry Potassium Hydrogen
DEVELOPMENT OF VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TAMSULOSIN HYDROCHLORIDE AND FINASTERIDE FROM TABLET DOSAGE FORM Susheel John Varghese‚ Leela Madhuri Pola‚ K.Murugavel‚ and T.K.Ravi. College of Pharmacy‚ Sri Ramakrishna Institute of Paramedical Sciences‚ Coimbatore – 641044‚ Tamilnadu. E-Mail: velmpharm24@gmail.com A precise‚ accurate and rapid RP-HPLC method has been developed and validated for the simultaneous estimation of Tamsulosin
Premium High performance liquid chromatography Chromatography Analytical chemistry
HPLC Theory: System Suitability Parameters High performance liquid chromatography is defined as a separation of mixtures of compounds due to differences in their distribution equilibrium between two phases‚ the stationary phase packed inside columns and the mobile phase‚ delivered through the columns by high pressure pumps. Components whose distribution into the stationary phase is higher‚ are retained longer‚ and get separated from those with lower distribution into the stationary phase. The
Premium Chromatography High performance liquid chromatography Analytical chemistry
Title : The Beer-Lambert Law and Its Limitation Objective : 1. To determine the linear relationship between absorbance and concentration of an absorbing species. 2. To study the effects of molecular dissociation complex formation on the applicability of the Beer-Lambert Law. 3. To investigate the derivation and limitation of Beer-Lambert Law. Introduction: In optics‚ the Beer–Lambert law‚ also known as Beer ’s law‚ the Lambert–Beer law‚ or the Beer–Lambert–Bouguer law relates the absorption
Premium Spectroscopy Concentration Electromagnetic radiation
Natural Products Experiment Niki Robinson 10008562 Practical 2: Identification and quantification of taxifolin from milk thistle Aim: to Identify and quantify the amount of taxifolin from three samples Silymarin stock solution‚ taxifolin and silybum marianum extract. ` Methodology: Using auto-pipette (20-100 microlitre)‚ pipetted 0.06 mg of Taxifolin was added to 10ml conical flask and made up to the mark (10ml) with H2O.This procedure was repeated with the same amounts foe silymarin stock
Premium High performance liquid chromatography Analytical chemistry Standard deviation