Affinity chromatography technique is used to separate proteins found in a mixture of solution. Affinity chromatography uses the strong interaction between a given protein and its corresponding molecule. In today’s lab‚ affinity chromatography was used to purify L-lactate dehydrogenase‚ which contains histidine-tagged protein. The histidine- tagged protein forms a strong interaction with the Ni-NTA column due to the presence of nickel ions. Varying concentration of imidazole was added to the column
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The History and Progression of Chemistry The birth of the modern atomic theory. In 1750‚ Rudjer Boscovich‚ a scientist born in what is now Croatia‚ suggested the theory that atoms were "uncuttable" might have been wrong. Boscovich thought that atoms contain smaller parts‚ which in turn contain still smaller parts‚ and so forth down to the fundamental building blocks of matter. He felt that these building blocks must be geometric points with no size at all. Today‚ most atomic physicists accept a
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The Complete Organic Chemistry Worksheet The Complete Organic Chemistry Worksheet.doc Name 1. Name the following hydrocarbons. 3 j’\- d\ m.u\ \q’\\Lxc.$’s CHr i"’ a.CH3-CH2-CH-CH-CH. I CH: 9n’ tt e. CHr-CH-CH-CH-CHl I t- f‚ ‚3‚ q - \c’ *"jtr1\\-s.x^t’"*- CHz I CH: CHr CHr CHr f. CH3-CH2-CH-CH-CH CHr ?"’ !t’ I j \- A.^ r.alh-{hq"{n-"* Ll A A \-k ‚e*q\t^’-tt i"’ f"l -’"’-lu*"‚Uo..q b cur-f-is-a"‚ "’ ll cH: g. CH: I CH2 3
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THE RULES AND REGULATIONS The following safety rules must be observed while performing any of the scheduled laboratory experiments in one of the Chemistry Laboratories. Students who are found in violation of the safety rules will be given a warning for the first offense‚ will be dismissed from the lab room for the second offense‚ and will be withdrawn from the class (lab as well as lecture) for the third offense. If a violation is observed at a particular lab table (such as spilled liquid - water
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Serena Contreras Advanced Chemistry period 2 Mrs. Horton
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AP Chemistry Lab 2: Analysis of Alum Lab Objective: In this lab we will analyze alum by two techniques in order to verify its identity. The melting point and the mole ratio of hydrated water to anhydrous aluminum potassium sulfate will be determined. Lab Procedure: 1. Use a mortar and pestle to crush alum. 2. Pack the alum in capillary tube‚ and then fasten it to the thermometer. 3. Fasten the thermometer to the ring stand. 4. Immerse the bottom of the
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AP Chemistry Period 1 Molecular Weight of a Condensable Vapor Lab Purpose: The purpose and objective of this lab was to find the molecular weight of a condensed vapor. Materials and Equipment: • Aluminum foil square (around 6cm on a side) • 125 mL flask • Barometer • 3 mL of unknown liquid • 200 mL graduated cylinder • 600 mL beaker • Pin • Balance (0.002g) • Bunsen Burner setup • Rubber band • Thermometer • Ceramic center wire gauze Procedure: 1. A 125 mL flask was obtained
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Polymer Chemistry Classification of Polymers – Notes prepared by The most common way of classifying polymers is to separate them into three groups - thermoplastics‚ thermosets‚ and elastomers5. The thermoplastics can be divided into two types - those that are crystalline and those that are amorphous. You may click on the words in the diagram below to learn more about these classifications. Thermoplastics Molecules in a thermoplastic are held together by relatively weak intermolecular
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Jamie Kim Mrs. Shaw Chemistry Honors 3 September 2014 Bubble Quality Lab Hypothesis: Bubble quality is most positively affected by the mixture of sugar and dish detergent due to the sticky consistency of sugar when emerged in liquid. Procedure: First of all‚ three cups were labelled according to their solution. Then‚ a teaspoon of dish soap and two-thirds of a cup of water was added and mixed to make cup#1. Next‚ a half teaspoon of table sugar was added to the same control solution and was
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Science Revision Notes Chemistry Atoms are the smallest particle of a particular element. Molecules are particles that contain 2 or more atoms joined together by chemical bonds. Elements are substances that are made up of only one type of atom. Compounds are substances form when two or more elements are joined chemically. Metals are solid at room temperature‚ with the exception of mercury. Metals are good conductors of electricity and heat. Metals can usually be melted at high temperatures. Non-metals
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