Transport of Solute in Solvent through Osmosis or Diffusion Due to Different Concentration Gradients Passing Through a Semi-permeable Membrane between Cell and Cells Environment Bio 101 Objective: The objective is to simulate passive transport: diffusion of solutes and osmosis of water through a semipermeable membrane (dialysis tubing). The experiment will show how molecules in solution move from areas of higher concentration to areas of lower concentration in the attempt to reach homeostasis
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Diffusion is one of several transport phenomena that occur in nature. A distinguishing feature of diffusion is that it results in mixing or mass transport without requiring bulk motion. Thus‚ diffusion should not be confused with convection or advection‚ which are other transport mechanisms that use bulk motion to move particles from one place to another. In Latin‚ "diffundere" means "to spread out". There are two ways to introduce the notion of diffusion: either a phenomenological approach starting
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* Diffusion-is the process whereby particles of liquids‚ gases‚ or solids intermingle as the result of their spontaneous movement caused by thermal agitation and in dissolved substances move from a region of higher to one of lower concentration. The rate of diffusion depends on the size of the surface area to the volume in the cell. The higher the surface area of cells in relation to their volume‚ the quicker diffusion takes place. Experiment on Diffusion Aim: to determine diffusion Materials:
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Physioex 9.0 Review Sheet Exercise 1 Cell Transport Mechanisms and Permeability Name Lab Time/Date ___ Activity 1 Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. Size of material and concentration 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? The molecules were too large to pass through. This is what I predicted
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Osmosis is the spontaneous net movement of solvent molecules through a partially permeable membrane into a region of higher solute concentration‚ in the direction that tends to equalize the solute concentrations on the two sides. It may also be used to describe a physical process in which any solvent moves‚ without input of energy‚ across a semipermeable membrane separating two solutions of different concentrations. Although osmosis does not require input of energy‚ it does use kinetic energy and
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Investigation: A factor that affects the movement of water in and out of cells. Osmosis: movement of water from a region of high water potential to a region of low water potential through a partially permeable membrane. High water potential = lots of free water molecules‚ low solute concentration Low water potential = very few free water molecules‚ high solute concentration Factors That Affect the Movement of Water in and out of Cells * Solute concentration * If one solution
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Osmosis: How fast can you move? The Rate at which Osmosis Occurs when Exposed to Various Toxicities Within the human body many things are occurring at all times. Without these small‚ seemingly insignificant processes all human life would cease to exist. One of the aforementioned activities is Osmosis‚ or the movement of water across a selectively permeable membrane eventually establishing equilibrium on both sides of the concentration gradient (Freeman 90). During this specific experiment involving
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DNA QUESTION 1995: L. PETERSON/AP BIOLOGY The diagram below shows a segment of DNA with a total length of 4‚900 base pairs. The arrows indicate reaction sites for two restriction enzymes (enzyme X and enzyme Y). (A) Explain how the principles of gel electrophoresis allow for the separation of DNA fragments. (B) Describe the results you would expect from electrophoretic separation of fragments from the following treatments of the DNA segment above. Assume that the digestion
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AP Lab Seven Genetics of Organisms Dan Torres AP Biology Block 1 January 24‚ 2011 Introduction & Background Fruit flies have made a huge contribution towards knowledge about genetics‚ but for most people‚ they are just annoying insects that are attracted to their fruit. Their scientific name is Drosophila melanogaster‚ and to scientists‚ they have been a key to understand many principles of heredity including sex linked inheritance‚ epistasis‚ multiple alleles‚ and gene mapping. Fruit
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Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 or 23 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through?50‚100‚ 200 4. Which materials diffused from the left beaker to the right beaker? Urea‚ NaCl and glucose diffused 5. Which did not? Why? Albumin was too large to diffuse into the right beaker. Activity 2: Simulating Dialysis 6. What happens to the urea concentration
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