BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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ABSTRACT Culture is the background of every human communication. Cultural embedding as a feature of texts in general is also valid in technical and scientific texts. As translation by humans is based on understanding‚ the translator needs knowledge in order to detect cultural aspects. This is possible by putting down implicit cultural references to certain structures on the text level. Cultural elements appear in the text on all levels – from the concept and form of words‚ to the sentence and text
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introduction to enzymes Enzymes are the foundation of energy and the life force in all living things. They are responsible for building‚ detoxifying‚ and healing the body. They are also the force that allows your body to digest and absorb food. Enzymes also regulate tens of thousands of other biochemical functions that take place in the body every day. Without enzymes‚ seeds would not sprout‚ fruit would not ripen‚ leaves would not change color‚ and life would not exist. Therefore‚ the study of enzymes has
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Enzyme Immobilization Methods Covalent Binding: Covalent binding is a conventional method for immobilization; it can be achieved by direct attachment with the enzyme and the material through the covalent linkage [37]. The covalent linkage is strong and stable and the support material of enzymes includes polyacrylamide‚ porous glass‚ agarose and porous silica [38]. Covalent method of immobilization is mainly used when a reaction process does not require enzyme in the product‚ this is the criteria
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The Effects of pH‚ Temperature‚ Enzyme‚ and Substrate Concentrations on Benzoquinone Production BIOL 2051 June 10th 2013 Introduction Enzymes are the ultimate catalysts of living things. Enzymes are made of proteins which are structured and directed by amino acids chains. Enzymes attract and fit substrate molecules to an active site. The active site binds the substrate molecules covalently to enzyme forming an enzyme-substrate complex‚ which catalyzes
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Computer Enzyme Action: Testing Catalase Activity 6A H2O2 is toxic to most living organisms. Many organisms are capable of enzymatically destroying the H2O2 before it can do much damage. H2O2 can be converted to oxygen and water‚ as follows: 2 H2O2 → 2 H2O + O2 Although this reaction occurs spontaneously‚ enzymes increase the rate considerably. At least two different enzymes are known to catalyze this reaction: catalase‚ found in animals and protists‚ and peroxidase‚ found in plants. A great
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Enzymes control the rate of metabolic reactions‚ they act as biological catalysts‚ which means they are used but not used up and they also control the speed of the reaction. Enzymes are proteins which means that anything that disrupts this structure such as high temperature or change in pH will affect the enzyme activity. There are many factors affecting enzyme action for example temperature effects them‚ if the Increasing the heat gives molecules more kinetic energy so they vibrate this can then
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Enzyme Lab: Peter Kuetzing – 10/4/2012 – F Block 1. How does changing the concentration of enzyme affect the rate of decomposition of H2O2? When more enzymes is added the rate of reaction speeds up. In this case‚ H2O2 will produce more O2‚ in return the kpa/min will go up. 2. What do you think will happen to the rate of reaction if the concentration of enzyme is increased to five drops? Predict what the rate would be for 5 drops. I think that the rate of reaction will slightly increase from
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Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include: background information about concepts involved in the lab‚ statement of purpose of the lab identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in the cells of the body
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BABS1201 Group Enzymes Project Group Protocol Including Equipment and Reagents List Lab Day: Wednesday Lab Time: 10am – 1pm Are you in Lab G20 (furthest from BSB Student Office) or Lab G21 (closest to BSB Student Office)?: Demonstrator Name: Daniel Winters Names of Group Members: Johnny Nguyen‚ Therese Pham‚ Linda Tang Name of Enzyme You are Investigating: Amylase Brief Background: Amylase is a digestive enzyme‚ produced mainly by the salivary glands and the pancreas‚ to break down
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